Back to results
US 2023/0145599 A1Published application
Microneedle Array Delivery of Adenovirus Vectored Vaccines With and Without Adjuvants
AssigneeUniversity of Pittsburgh - Of the Commonwelth System of Higher Education
InventorsLouis Falo, Jr., Andrea A. Gambotto, Geza Erdos
Life of this patent
Estimating the term…
Abstract
Provided herein are microneedle array devices for delivery of recombinant adenovirus particles, methods of making the devices, and used for the devices. The microneedle array devices are storage-stable at 4° C., retaining adenovirus infectivity for at least one month.
Claims32
A microneedle device, comprising:
- a backing layer;
- and
- a plurality of microneedles extending from the backing layer, and comprising a dissolvable (aqueous-soluble) and/or bioerodible matrix comprising trehalose, and a recombinant adenovirus particle comprising a gene for expressing a polypeptide or RNA.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
27-31. (canceled)
(canceled)
A method of forming a microneedle device, comprising:
- forming or providing a production mold of a flexible material, the production mold comprising a plurality of cavities that are shaped to define a plurality of respective microneedles having a stem, a head, a filleted base, and at least one undercut feature, the microneedles optionally having a length of 1 mm or less;
- delivering a first dissolvable or bioerodible material comprising trehalose into at least the microneedle head portion defined by the respective cavities of the production mold, and prior to or during delivery of the first dissolvable or bioerodible material into at least the microneedle head portion, incorporating a recombinant adenovirus particle comprising a gene for expressing a polypeptide or RNA into the first dissolvable or bioerodible material to produce a dissolvable or biodegradable matrix;
- delivering the first dissolvable or bioerodible material and/or one or more additional dissolvable or bioerodible materials into the cavity and forming a plurality of microneedles in the production mold that include the dissolvable or biodegradable matrix;
- and
- removing the microneedles from the production mold by pulling the microneedles out of the mold,
- wherein the flexible material of the production mold has sufficient elasticity to allow for the molded microneedle array to be removed from the production mold, e.g., in a single pull, without damaging the integrity of the shape of the microneedles as defined by the mold.
3 more elements
36-44. (canceled)
Dependencies are inferred from the claim text.