Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
Assignee10x Genomics, Inc.
InventorsJessica Michele Terry, Tobias Daniel Wheeler
Life of this patent
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Abstract
Provided herein are methods, compositions, and systems for multiplexed analysis of individual cells or cell populations. Cells encapsulated in beads and/or biomolecules are sequentially co-partitioned, allowing for analysis of two different types of biomolecules (e.g., RNA and DNA). The present invention leverages different polymer dissociation mechanisms, accompanied with barcoding of biomolecules (e.g., nucleic acid molecules) for multiplexed measurements in single cells. Sequential co-partitioning and barcode technology enables identification and quantitation of DNA and RNA from single cells.
Claims20
1-37. (canceled)
A method for barcoding nucleic acid molecules of a cell, comprising:
- (a) generating a cell bead comprising the cell, wherein the cell comprises a first nucleic acid molecule and a second nucleic acid molecule, and wherein the first nucleic acid molecule and the second nucleic acid molecule are different;
- (b) providing a first support comprising a first nucleic acid barcode sequence and generating a first barcoded nucleic acid molecule comprising (i) the first nucleic acid barcode sequence, and (ii) a sequence of the first nucleic acid molecule;
- (c) providing a second support comprising a second nucleic acid barcode sequence that is different than the first nucleic acid barcode sequence and generating:
- a second barcoded nucleic acid molecule comprising (i) the second nucleic acid barcode sequence, and (ii) a sequence of the second nucleic acid molecule, wherein the second nucleic acid barcode sequence is different from the first nucleic acid barcode sequence;
- and
- a third barcoded nucleic acid molecule comprising (i) the first nucleic acid barcode sequence;
- and (ii) the second nucleic acid barcode sequence.
Dependencies are inferred from the claim text.