Compositions for treatment of spinal cord injury, methods and uses thereof
AssigneesUniversidade Do Minho, B'acis-AssociaçãO CiêNcia, InovaçãO E SAúDe-Braga
InventorsAntónio José BRAGA OSÓRIO GOMES SALGADO, Nuno André MARTINS SILVA, Rita Catarina ASSUNÇÃO RIBEIRO SILVA, Eduardo DOMINGOS CORREIA GOMES, Luisa PINTO, Sofia Cristina CRAVINO SERRA, Jorge Miguel CIBRÃO RIBEIRO, Susana Isabel GONÇALVES MONTEIRO, Andreia Filipa GOMES DE PINHO
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to a method and composition for the treatment or therapy of spinal cord injury, the composition comprising a total secretome, obtainable from mesenchymal stem cells. The composition is suitable for systemic delivery, preferably intravenous systemic delivery.
Claims16
A method of treating a patient with an acute spinal cord injury, the method comprising administering to the patient a composition comprising a secretome obtained from mesenchymal stem cells, wherein the mesenchymal stem cells are adipose tissue-derived stem cells, wherein the composition is a single dose injectable composition and wherein the composition improves locomotor symptoms in the patient.
(canceled)
A method for obtaining a secretome from adipose-derived stem cells comprising the following steps:
- obtaining the adipose-derived stem cells;
- culturing the adipose-derived stem cells at a density of 4000 cells/cm2 and maintaining the adipose-derived stem cells in culture for 24-96 h, in a suitable medium;
- washing the adipose-derived stem cells with phosphate buffered saline;
- washing the adipose-derived stem cells with basal cell culture media for neuronal cell culture supplemented with a suitable antibiotic;
- culturing the adipose stem cells in the basal cell culture media over 24 h;
- collecting and centrifuging the basal cell culture media to remove debris;
- and
- concentrating the collected secretome.
Dependencies are inferred from the claim text.