Treatment of covid-19 with reverse micelle system comprising unmodified oligonucleotides
AssigneesMedesis Pharma, Centre National De La Recherche Scientifique, Universite De Montpellier
InventorsJean-Claude MAUREL, Abdelkader MOURI, Hervé SEITZ, Sophie MOCKLY
Life of this patent
Estimating the term…
Abstract
The present invention relates to specific reverse micelle system of the invention which allows the administration and intracellular delivery of unmodified oligonucleotide, such as siRNA, targeting one or more genes of the SARS-CoV-2 virus. The reverse micelle system of the invention is thus particularly useful for the treatment of the viral pathology linked to the SARS-CoV-2 virus.
Claims20
A reverse micelle system comprising at least one sterol, acylglycerol, phospholipid, an alcohol, water and at least one unmodified oligonucleotide targeting one or more genes of SARS-CoV-2 virus.
The reverse micelle system according to claim 8, wherein the siRNA presents a guide strand which comprises, or consists of, one of the following sequences:
- SEQ ID NO:
- 1:
- 5′ P-UGAUAGUAGUCAUAAUCGCUA 3′;
- SEQ ID NO:
- 3:
- 5′ P-UGACUUAAAGUUCUUUAUGCG 3′;
- SEQ ID NO:
- 5:
- 5′ P-UUAGCUAAAGACACGAACCGG 3′.
- SEQ ID NO:
- 8:
- 5′ P-UGACUUAAAGUUCUUUAUGCUC 3′;
- SEQ ID NO:
- 10:
- 5′ P-UAUAGCUAAAGACACGAACCC 3′;
- SEQ ID NO:
- 11:
- 5′ P-AUAGCUAAAGACACGAACCGG 3′;
- SEQ ID NO:
- 12:
- 5′ P-UUGAGUGCAUCAUUAUCCAAG 3′;
- SEQ ID NO:
- 13:
- 5′ P-CUUGACUGCCGCCUCUGCUCG 3′;
- SEQ ID NO:
- 14:
- 5′ P-GUUGAGUGCAUCAUUAUCCAC 3′;
- or
- SEQ ID NO:
- 15:
- 5′ P-UCCUGAUUAUGUACAACACCG 3′.[[;]]
A siRNA presenting a guide strand which comprises, or consists of, one of the following sequences:
- SEQ ID NO:
- 1:
- guide strand:
- 5′ P-UGAUAGUAGUCAUAAUCGCUA 3′;
- SEQ ID NO:
- 3:
- guide strand:
- 5′ P-UGACUUAAAGUUCUUUAUGCG 3′;
- SEQ ID NO:
- 5:
- guide strand:
- 5′ P-UUAGCUAAAGACACGAACCGG 3′;[[.]]
- SEQ ID NO:
- 8:
- guide strand:
- 5′ P-UGACUUAAAGUUCUUUAUGCUC 3′;
- SEQ ID NO:
- 10:
- guide strand:
- 5′ P-UAUAGCUAAAGACACGAACCC 3′;
- SEQ ID NO:
- 11:
- guide strand:
- 5′ P-AUAGCUAAAGACACGAACCGG 3′;
- SEQ ID NO:
- 12:
- guide strand:
- 5′ P-UUGAGUGCAUCAUUAUCCAAG 3′;
- SEQ ID NO:
- 13:
- guide strand:
- 5′ P-CUUGACUGCCGCCUCUGCUCG 3′;
- SEQ ID NO:
- 14:
- guide strand:
- 5′ P-GUUGAGUGCAUCAUUAUCCAC 3′;
- or
- SEQ ID NO:
- 15:
- guide strand:
- 5′ P-UCCUGAUUAUGUACAACACCG 3′.[[;]]
A siRNA duplex, with guide strand and passenger strand, which comprises, or consists of, one of the following duplex sequences:
- siRNA no 1
- SEQ ID NO:
- 1:
- guide strand:
- 5′ P-UGAUAGUAGUCAUAAUCGCUA 3′;
- SEQ ID NO:
- 2:
- passenger strand:
- 5′ GCGAUUAUGACUACUAUUUUA 3′;[[.]]
- siRNA no 2
- SEQ ID NO:
- 3:
- guide strand:
- 5′ P-UGACUUAAAGUUCUUUAUGCC 3′;
- SEQ ID NO:
- 4:
- passenger strand:
- 5′ CAUAAAGAACUUUAAGUCCUC 3′;[[.]]
- siRNA no 3
- SEQ ID NO:
- 5:
- guide strand:
- 5′ P-UUAGCUAAAGACACGAACCGG 3′;
- SEQ ID NO:
- 6:
- passenger strand:
- 5′ GGUUCGUGUCUUUAGCUACUC 3′;[[.]]
- or
- siRNA no 4
- SEQ ID NO:
- 8:
- guide strand:
- 5′ P-UGACUUAAAGUUCUUUAUGCUC 3′;
- SEQ ID NO:
- 9:
- passenger strand:
- 5′ GCAUAAAGAACUUUAAGUUUCU 3′.
Dependencies are inferred from the claim text.