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US 2023/0144097 A1Published application
Active dna transposon systems and methods for use thereof
AssigneeInstitute of Zoology, Chinese Academy of Sciences
InventorsHaoyi WANG, Yong ZHANG, Shengjun TAN, Tongtong ZHANG
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Abstract
Provided are engineered transposable elements, gene transfer systems comprising the engineered transposable elements, as well as methods and kits for using the same. The compositions, systems, and methods are useful for inserting a heterologous nucleic acid into a target nucleic acid in vitro or in a cell.
Claims25
An engineered transposable element comprising, from 5′ to 3′:
- a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR);
- wherein the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 1-26 and 79-90, a variant thereof, or a fragment thereof, wherein the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 27-52 and 91-102, a variant thereof, or a fragment thereof;
- and
- wherein the engineered transposable element exhibits transposition activity that allows the heterologous nucleic acid to be inserted into a DNA of a cell.
3 more elements
(canceled)
12-13. (canceled)
A gene transfer system comprising:
- i) an engineered transposable element;
- and ii) a transposase, or a nucleic acid encoding h transposase, wherein the engineered transposable element comprises from 5′ to 3′:
- a 5′TR, a heterologous nucleic acid, and a 3′TR,
- wherein the engineered transposable element exhibits transposition activity that allows the heterologous nucleic acid to be inserted into a DNA of a cell, and
- wherein the transposase comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 53-78 and 103-114, or a variant thereof.
3 more elements
20-24. (canceled)
(canceled)
28-31. (canceled)
33-39. (canceled)
Dependencies are inferred from the claim text.