Microbeads and uses thereof
AssigneeShanghai Polaris Biology Co., Ltd.
InventorsRan Wei, Shuangwu Sun, Yuchong Wang, Ziyang Ge
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Abstract
The present disclosure provides a microbead and a method for preparing the same. Also provided is a method for detecting and analyzing biomolecules in sample by using element analysis.
Claims27
1-64. (canceled)
A population of microbeads comprising two or more subpopulations of the microbeads, wherein at least one of the microbeads comprises a plurality of functional groups on surface of a microbead substrate, a first subset of the plurality of the functional groups is attached to a chelating group chelated with an element tag, and a second subset of the plurality of the functional groups is coupled with an affinity moiety capable of binding to a target molecule;
- and wherein at least one subpopulation of the microbeads comprises an element tag that is different from at least another subpopulation of the microbeads, and at least one subpopulation of the microbeads comprises an affinity moiety that is different from another subpopulation of the microbeads.
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73-97. (canceled)
A method of performing a multiplex analysis of two or more analytes in a sample, comprising:
- (a) contacting a population of microbeads with the sample, wherein at least one of the microbeads comprises a plurality of functional groups on surface of the microbead substrate;
- a first subset of the plurality of the functional groups is attached to a chelating group chelated with an element tag;
- a second subset of the plurality of the functional groups is coupled with an affinity moiety capable of binding to an analyte of the two or more analytes, wherein at least one subpopulation of the microbeads comprises the element tag that is different from at least another subpopulation of the microbeads, and at least one subpopulation of the microbeads comprises the affinity moiety that is different from another subpopulation of the microbeads,
- (b) incubating the population of the microbeads with two or more detecting reagents, wherein each of the detecting reagents allows for detection of a microbead bound to an analyte;
- (c) simultaneously analyzing the two or more analytes in the sample.
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111-113. (canceled)
A kit comprising:
- (1) an instruction for performing a multiplex analysis of two or more analytes by using the kit,
- (2) a population of microbeads comprising two or more subpopulations of the microbeads, wherein at least one of the microbeads comprises a plurality of functional groups on surface of a microbead substrate, a first subset of the plurality of the functional groups are attached to a chelating group chelated with an element tag, and a second subset of the plurality of the functional groups is coupled with an affinity moiety capable of binding to a target molecule, and wherein at least one subpopulation of the microbeads comprises an element tag that is different from at least another subpopulation of the microbeads, and at least one subpopulation of the microbeads comprises an affinity moiety that is different from another subpopulation of the microbeads, and
- (3) two or more detecting reagents, wherein each of the detecting reagents comprises a detecting element tag and specifically binds to an analyte of the two or more analytes.
Dependencies are inferred from the claim text.