Pin6 proteins for the formation of nodule-like structures
OwnerFranck Anicet Ditengou, Thomas Ottinventors
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Abstract
The present disclosure provides DNA molecules and constructs, including their nucleotide sequences, useful for expressing proteins in plants to promote formation of nodule-like structures in the presence of Rhizobia. The present disclosure also provides DNA molecules and constructs, including their nucleotide sequences, useful for expressing proteins in plants to increase drought resistance. The present disclosure also provides plants and plant cells transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules, along with methods of their use.
Claims30
A recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a polynucleotide segment encoding an auxin transporter protein or fragment thereof, wherein:
- a. said protein comprises the amino acid sequence of any of SEQ ID NOs:
- 3 and 5-14;
- b. said protein comprises an amino acid sequence having at least 85%, or 90%, or 95%, or 98% or 99%, or about 100% amino acid sequence identity to any of SEQ ID NOs:
- 3 and 5-14;
- c. said polynucleotide segment hybridizes under stringent hybridization conditions to a polynucleotide having the nucleotide sequence of any of SEQ ID NO:
- 1, 2, or 4;
- or
- d. said polynucleotide segment comprises the nucleotide sequence of any of SEQ ID NO:
- 1, 2, or 4.
A method of detecting the presence of a protein, or fragment thereof, in a sample comprising protein, wherein said protein comprises the amino acid sequence of any of SEQ ID NOs:
- 3 or 5-14;
- or said protein comprises an amino acid sequence having at least 85%, or 90%, or 95%, or 98% or 99%, or about 100% amino acid sequence identity to any of SEQ ID NOs:
- 3 or 5-14;
- comprising:
- a. contacting said sample with an immunoreactive antibody;
- and
- b. detecting the presence of said protein, or fragment thereof.
Dependencies are inferred from the claim text.