Beta-ketoacyl-acp synthase iv variants
AssigneeCorbion Biotech, Inc.
InventorsJoshua FERREIRA, Janice Lau WEE, Nien-Hsi KO
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Abstract
Provided are non-natural or variant β-ketoacyl-acyl carrier protein (ACP) synthase (KAS) IVa enzymes (KASIVa), polynucleotides encoding such variant KASIVa, host cells expressing such variant KASIVa, oils and oil products produced by such cells, and methods of making and using such variant KASIVa.
Claims91
A polynucleotide encoding a β-ketoacyl-acyl carrier protein (ACP) synthase (KAS) IVa enzyme (KASIVa) variant, wherein the KASIVa variant comprises at least about 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity to amino acid residues 34-523 of SEQ ID NO:
- 4 and comprises an X at the position corresponding to position 146;
- wherein X is an amino acid residue selected from the group consisting of glycine (G), asparagine (N) and serine (S), wherein the position is with reference to SEQ ID NO:
- 4, and wherein the KASIVa variant catalyzes the elongation of a medium-chain fatty acyl-ACP.
A non-natural KASIVa variant comprising a polypeptide sequence having at least about 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identity to amino acid residues 34-523 of SEQ ID NO:4 and comprises an X at the position corresponding to position 146, wherein X is an amino acid residue selected from the group consisting of glycine (G), asparagine (N), serine (S), wherein the positions are with reference to SEQ ID NO:
- 4.
A microbial oil comprising triglycerides, wherein the oil comprises at least about 35%, at least about 40%, at least about 41%, at least 42%, at least about 43%, or at least about 44% tridecanoin.
Dependencies are inferred from the claim text.