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US 2023/0143790 A1Published application
Method for the aerobic and anaerobic cultivation of microorganisms, method for the production of a preparation for cleaning radioactive liquids and radioactively charged surfaces, method for cleaning radioactive liquids and method for cleaning radio-actively charged surfaces
AssigneeSylvia SCHREIBER
InventorsAlbina Aleksandrovna KORNILOVA, Vladimir Ivanovich VYSOTSKII
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Abstract
The invention relates to a method for aerobic and anaerobic cultivation of microorganisms. The invention also relates to a method for producing a preparation for cleaning radioactive liquids and radioactively charged surfaces. Likewise, the invention further relates to a method for cleaning radioactive liquids and radioactively charged surfaces.
Claims22
A method for the aerobic cultivation of microorganisms, comprising the steps of:
- providing an aqueous solution having a pH value of 5.5 to 9.0 in a container,
- adding a substrate in a first substrate dosage to the aqueous solution,
- adding further elements to the aqueous solution,
- adding an inoculant with microorganisms to the aqueous solution,
- wherein an initial product is provided by the abovementioned steps, and wherein, subsequently, the following steps are performed:
- introducing air into the initial product or into intermediate products developing therefrom,
- varying a temperature of the initial product or the intermediate products developing therefrom in a range from 15 to 35° C.,
- wherein, after having added the substrate in the first substrate dosage, the following steps are performed for monitoring the cultivation:
- taking a reference liquid sample and determining a first concentration of organic substance in the reference liquid sample,
- taking another liquid sample and determining another concentration of organic substance in the further liquid sample after the expiration of the first waiting time,
- if the further concentration of organic substance is smaller than 10 percent of the first concentration of organic substance, adding substrate in another substrate dosage,
- repeating the abovementioned steps for monitoring the cultivation until a sufficient amount of biomass is present in the container.
9 more elements
(canceled)
A method for cleaning radioactive liquids, comprising the steps of:
- providing radioactive liquid to be cleaned in a reactor,
- adding a preparation for cleaning radioactive liquids into the reactor, the preparation produced by a method for the production of a perparation for cleaning radioactive liquids and surfaces comprising
- providing of a substance with aerobic microorganisms, particularly cultivated comprising the steps of:
- providing an aqueous solution having a pH value of 5.5 to 9.0 in a container
- adding a substrate in a first substrate dosage to the aqueous solution,
- adding further elements to the aqueous solution,
- adding an inoculant with microorganisms to the aqueous solution,
- wherein an initial product is provided by the abovementioned steps, and wherein, subsequently, the following steps are performed:
- introducing air into the initial product or into intermediate products developing therefrom,
- varying a termperature of the initial product or the intermediate products developing therefrom in a range from 15 to 35 degrees Celsuis,
- wherein, after having added the substratae in the first substrate dosage, the following steps are performed for monitoring the cultivation:
- taking a reference liquid sample and determining a first concentration of organic substance in the reference liquid sample, substance in the reference liquid sample,
- taking another liquid sample and determining another concentration of organic substance in the further liquid sample after the expiration of the first waiting time,
- if the further concentration of organic substance is smaller than 10 percent of the first concentration of organic susbtance, adding substrate in another substrate dosage,
- repeating the abovementioned steps for monitoring the cultivation until a sufficient amount of biomass is present in the container, or
- providing of a substance with anaerobic microorganisms, particularly cultivated the anaerobic cultivation of microorganisms comprising the steps of:
- providing an aqueous solution having a pH value of 4.5 to 7.5 in a container,
- adding a substrate in a first substrate dosage to the aqueous solution,
- adding further elements to the aqueous solution,
- adding an inoculant with microorganisms to the aqueous solution, wherein an initial product is provided by the abovementioned steps, and wherein, susbsequently, the following steps are performed:
- hermetically sealing the container,
- varying a temperature of the initial product or the intermediate products developing therefrom in a range from 40 to 80° C.,
- wherein, after having added the substrate in the first substrate dosage, the following steps are performed for monitoring the cultivation:
- taking a reference liquid sample and determining a first concentration of organic substance in the reference liquid sample,
- taking another liquid sample and determining another concentration of organic substance in the further liquid sample after the expiration of the first waiting time,
- if the further concentration of organic substance is smaller than 10 percent of the first concentration of organic substance, adding substrate in another substrate dosage,
- repeating the abovementioned steps for monitoring the cultivation until a sufficient amount of biomass is present in the container.
- wherein the substance is subjected to the following steps for washing:
- centrifuging the substance for producing wet biomass.
- adding an isotonic solution to the wet biomass for producing a washed substance, wherein the steps for washing are repeated a plurality of times, if required, to thereby provide the preparation for cleaning radioactive liquids and surfaces,
- adding a substrate into the reactor,
- adding elements selected from the group of K, Ca, P, S, N, Zn, Co, Mn, Cl, Cu, Mo, Ni, Se, B, Fe,
- wherein, by the abovementioned steps, a working medium is provided, and wherein, further, the following step is performed:
- removing cleaned radioactive liquid from the reactor.
31 more elements
22-32. (canceled)
Dependencies are inferred from the claim text.