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US 2023/0143626 A1Published application
Fast and robust bi-functional mutants of taq dna polymerase
AssigneeDna Polymerase Technology, Inc.
InventorsMilko B. Kermekchiev, Zhian Zhang
Life of this patent
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Abstract
Amino acid substitutions that provide for increased elongation rates, resistance to PCR inhibitors, and reverse transcriptase activity of Thermus aquaticus (Taq) DNA polymerase enzymes are provided. Also provided are related methods of using the Taq DNA polymerase enzymes to rapidly detect nucleic acids of interest in crude biological samples, without DNA/RNA extraction.
Claims20
A DNA polymerase comprising a polypeptide sequence having at least 95% sequence identity to positions 279 through 832 of SEQ ID NO:
- 1, and further comprising one or more amino acid substitutions selected from the group consisting of A391T, P752S, A814T, and combinations thereof, wherein all amino acid substitutions are relative to SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.