METHODS OF USING A TGF-beta KNOCKOUT CELL LINE AND COMPOSITIONS RESULTING THEREFROM
AssigneeBio-Techne Corporation
InventorsAnthony D. Person, John E. Humphrey
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Abstract
This disclosure describes protein compositions including minimal TGFβ1 contamination, methods for making those protein compositions, and methods for using those protein compositions. In one aspect, this disclosure describes protein compositions produced by a TGFβ1 knockout cell line and methods for using those compositions including, for example, in research or as a therapeutic or prophylactic. In another aspect, this disclosure describes a TGFβ1 knockout cell line and methods of making that cell line.
Claims20
A method for producing a target biologic, wherein the method comprises:
- culturing a TGFβ1 knockout cell line that overexpresses the target biologic;
- and
- purifying the target biologic.
A composition comprising a target biologic,
- wherein the target biologic comprises a TGFβ superfamily ligand, and
- wherein the composition comprises less than 0.5 ng/mL TGFβ1.
A composition comprising a target biologic,
- wherein the target biologic comprises a biologic drug, and
- wherein the composition comprises less than 0.5 ng/mL TGFβ1.
Dependencies are inferred from the claim text.