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US 2023/0143486 A1Published application
Methods of generating midbrain dopamine neurons, midbrain neurons and uses thereof
AssigneeMemorial Sloan Kettering Cancer Center
InventorsLorenz STUDER, Tae-Wan KIM, So-Yeon KOO
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Abstract
The present disclosure provides methods for generating midbrain dopamine neurons and precursors thereof, midbrain dopamine neurons and precursors thereof generated by such methods and compositions comprising such cells, and uses thereof for preventing, modeling, and/or treating a neurological disorder.
Claims82
An in vitro method for inducing differentiation of stem cells, comprising:
- contacting the stem cells with at least one inhibitor of Small Mothers Against Decapentaplegic (SMAD) signaling, at least one activator of Sonic hedgehog (SHH) signaling, and at least one activator of wingless (Wnt) signaling;
- and
- contacting the cells with at least one activator of fibroblast growth factor (FGF) signaling and at least one inhibitor of Wnt signaling to obtain a population of differentiated cells expressing at least one marker indicating a midbrain dopamine neuron or a precursor thereof.
A kit for inducing differentiation of stem cells to midbrain dopamine neurons or precursors thereof, comprising:
- (a) at least one inhibitor of SMAD signaling;
- (b) at least one activator of SHH signaling;
- (c) at least one activator of Wnt signaling;
- (d) at least one inhibitor of Wnt signaling;
- and
- (e) at least one activator of FGF signaling.
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Dependencies are inferred from the claim text.