Method for direct microbial identification
AssigneeQuest Diagnostics Investments Incorporated
InventorsErik P. Johnson, Nik Isely, Jamie L. Platt, Martin Siaw, Ron M. Kagen, Dale A. Schwab
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Abstract
Described herein are methods for direct detection of microbial agent(s) in a polymicrobial sample, such as a biological sample from a human, without culturing the microbial agent(s). The direct detection can identify mixtures of bacteria and/or fungi in the sample. Also described are primer sequences and amplification techniques for performing the direct detection methods.
Claims23
-20. (canceled)
A kit for determining the presence of one or more microbial agents in a polymicrobial sample without culturing the microbial agents, comprising at least one oligonucleotide primer selected from the group consisting of SEQ ID NOs:
- 1-335.
The kit of claim 210, wherein the adapter sequence is selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2.
A kit comprising an amplification reaction mixture, wherein the amplification reaction mixture comprises primers that specifically amplify at least one target sequence of bacterial 16S rDNA, at least one target sequence of fungal ITS rDNA, and at least one target sequence from each of Mycobacterium rpoB, Staphylococcus rpoB, Streptococcus rpoB, Burkholderia recA, Enterococcus tuf and Pseudomonas gvrB.
Dependencies are inferred from the claim text.