A method of single-stranded rna purification
AssigneeSartorius BIA Separations d.o.o.
InventorsSpela PERSIC, Urh CERNIGOJ, Darko DOLENC, Peter S. GAGNON
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Abstract
A method of single-stranded RNA purification comprising the steps: applying a sample containing single-stranded RNA to a solid phase dominantly or exclusively bearing both primary and secondary amino groups on its surface under conditions sufficient to bind at least predominantly the single-stranded RNA, eluting the single-stranded RNA adsorbed on the surface of the solid phase from the solid surface by means of an elution buffer having a higher concentration of comprising soluble pyrophosphate anions than needed for desorbing double-stranded RNA.
Claims21
A method of single-stranded RNA purification comprising the steps:
- applying a sample containing single-stranded RNA to a solid phase dominantly or exclusively bearing both primary and secondary amino groups on its surface under conditions sufficient to bind at least predominantly the single-stranded RNA,
- eluting the single-stranded RNA adsorbed on the surface of the solid phase from the solid surface by means of an elution buffer having a pyrophosphate concentration in the range of 1 mM to 1000 mM.
13-14. (canceled)
A method of single-stranded RNA purification comprising the steps:
- applying a sample containing single-stranded RNA at a pH in the range of 5.5 to 8.5 to a solid phase dominantly or exclusively bearing both primary and secondary amino groups on its surface,
- eluting the single-stranded RNA adsorbed on the surface of the solid phase from the solid surface by means of an increasing gradient of an elution buffer having a pyrophosphate concentration in the range of 10 mM to 150 mM.
Dependencies are inferred from the claim text.