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US 2023/0142476 A1Published application
Kidney organoids and method for producing the same
AssigneesPostech Research and Business Development Foundation, Unist (Ulsan National Institute of Science and Technology)
InventorsDong Sung Kim, Tae Eun Park, Hyeon Ji Lim, Do Hui Kim
Life of this patent
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Abstract
Disclosed is a method for producing kidney organoids including steps of: (1) differentiating stem cells into metanephric mesenchyme cells; (2) forming metanephric mesenchyme cell aggregates by culturing the metanephric mesenchyme cells; and (3) differentiating the metanephric mesenchyme cell aggregates into kidney organoids.
Claims10
A method for producing kidney organoids comprising steps of:
- (1) differentiating stem cells into metanephric mesenchyme cells;
- (2) forming metanephric mesenchyme cell aggregates by culturing the metanephric mesenchyme cells;
- and
- (3) differentiating the metanephric mesenchyme cell aggregates into kidney organoids.
1 more element
A kidney organoid differentiated from a metanephric mesenchyme cell aggregate formed under a hypoxic condition,
- wherein an expression level of at least one of mRNA corresponding to proximal tubules, mRNA corresponding to Henle loops, or mRNA corresponding to distal tubules in the kidney organoid is higher than that in a kidney organoid differentiated from a metanephric mesenchyme cell aggregate formed under a normoxic condition,
- the hypoxic condition is a condition with an oxygen concentration of more than 1% to less than 10%, and
- the normoxic condition is a condition with an oxygen concentration of 10% or more.
Dependencies are inferred from the claim text.