Compensation of intensity variances in images used for colony enumeration

A system, comprising:

• a processor;
• and
• a memory storing computer program instructions which when executed by the processor cause the processor to perform operations comprising:
• determine an area of interest on a growth media;
• calculate an overall brightness control value for a plurality of illumination sources configured to illuminate the growth media, the overall brightness control value generating at least one image that substantially matches a target intensity at the area of interest;
• calculate an individual brightness value for each illumination source of the plurality of illumination sources based on the overall brightness control value by individually adjusting a brightness of each illumination source to generate at least one image that substantially matches the target intensity in each respective illumination source's area of influence;
• and
• determine a calibrated brightness value for each illumination source based on an image intensity with each illumination source turned on at the respective individual brightness value and an intensity that each illumination source generates within each respective area of influence when turned on alone .

A non-transitory computer readable medium storing computer program instructions for designing microstructures, the computer program instructions when executed by a processor cause the processor to perform operations comprising:

• determining an area of interest on a growth media;
• calculating an overall brightness control value for a plurality of illumination sources configured to illuminate the growth media, the overall brightness control value generating at least one image that substantially matches a target intensity at the area of interest;
• calculating an individual brightness value for each illumination source of the plurality of illumination sources based on the overall brightness control value by individually adjusting a brightness of each illumination source to generate at least one image that substantially matches the target intensity in each respective illumination source's area of influence;
• and
• determining a calibrated brightness value for each illumination source based on an image intensity with each illumination source turned on at the respective individual brightness value and an intensity that each illumination source generates within each respective area of influence when turned on alone.