Targeted gene modification using oligonucleotide-mediated gene repair
AssigneesCibus US LLC, Cibus Europe B.V.
InventorsPeter R. BEETHAM, Gregory F.W. GOCAL, Christian SCHOPKE, Noel Joy SAUER, James PEARCE, Rosa E. SEGAMI, Jerry MOZORUK
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Abstract
The invention provides to improved methods for the modification of genes in plant cells, and plants and seeds derived therefrom. More specifically, the invention relates to the increased efficiency of targeted gene mutation by combining gene repair oligonucleotides with approaches that enhance the availability of components of the target cell gene repair mechanisms.
Claims1
A method for introducing a gene repair oligonucleobase (GRON)-mediated mutation into a target deoxyribonucleic acid (DNA) sequence in a cell, comprising:
- culturing the cell under conditions that increase one or more cellular DNA repair processes prior to, and/or coincident with, delivery of a GRON into the plant cell.
Dependencies are inferred from the claim text.