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US 2023/0141569 A1Published application
Fluorescent dye, and preparation method and uses thereof
AssigneeFluorescence Diagnosis (Shanghai) Biotech Company
InventorDasheng ZHANG
Life of this patent
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Abstract
A fluorescent dye, a preparation method and uses thereof, wherein the fluorescent dye has the advantages of sensitivity and specificity to viscosity response, low background fluorescence and the like, and can also be used as a fluorescence activated and lightened-up probe for fluorescence labeling, quantification or detection of proteins, enzymes or nucleic acids.
Claims12
1-10. (canceled)
A fluorescent dye, which is shown as Formula (I),
- wherein:
- Ar is arylene or heteroarylene, and optionally, hydrogen atoms in Ar are independently substituted by halogen atoms;
- D- is HO— or N(X1)(X2)—, and X1 and X2 are independently selected from hydrogen, alkyl or modified alkyl;
- X1 and X2 are optionally interconnected, and form an alicyclic heterocycle with N atoms;
- and X1 and X2 are optionally and independently form an alicyclic heterocycle with Ar;
- Y is O or S;
- R1 is hydrogen or alkyl;
- and
- R2 is a halogen atom, —OH or —CN;
- wherein:
- the “alkyl” is C1-C10 straight or branched alkyl;
- the “modified alkyl” is independently a group obtained by replacing any carbon atom in C1-C16 straight or branched alkyl with one or more groups selected from halogen atom, —OH, —CO—, —O—, —CN, —SO3H—, primary amino group, secondary amino group and tertiary amino group, or the carbon-carbon single bond is optionally and independently replaced by a carbon-carbon double bond or a carbon-carbon triple bond;
- the replacement of carbon atoms refers to that carbon atoms or the carbon atoms and hydrogen atoms thereon together are replaced by a corresponding group;
- the “halogen atom” is independently F, Cl, Br or I;
- the “alicyclic heterocycle” is a saturated or unsaturated 4- to 15-membered monocyclic or polycyclic alicyclic heterocycle containing one or more heteroatoms of N, O, S or Si on the ring, and the alicyclic heterocycle containing S heteroatom(s) on the ring include the ones that contain —S—, —SO— or —SO2— on the ring;
- the alicyclic heterocycle is optionally substituted by a halogen atom, an alkyl, an aryl or a modified alkyl;
- the “arylene” is independently a 5- to 13-membered monocyclic or dicyclic or fused dicyclic or fused polycyclic subaromatic group;
- the “heteroarylene” is independently a 5- to 13-membered monocyclic or dicyclic or fused dicyclic or fused polycyclic sub-heteroaromatic group containing one or more heteroatoms selected from N, O, S or Si on the ring;
- the “primary amino group” is R′NH2 group;
- the “secondary amino group” is R′NHR″ group;
- the “tertiary amino group” is R′NR″R′″ group;
- each R′, R″, R″ is independently a single bond, hydrogen, alkyl, or alkylene;
- the “alkylene” is C1-C10 straight or branched alkylene.
20 more elements
Dependencies are inferred from the claim text.