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US 2023/0141310 A1Published application
Human gut microbiome-derived biosynthetic enzymes for production of fatty acid amides
AssigneeMassachusetts Institute of Technology
InventorsChristopher A. Voigt, Fang-Yuan Chang
Life of this patent
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Abstract
Disclosed herein, in some embodiments, are vectors encoding biosynthetic enzymes from gut microbiome-derived bacterium (e.g., Clostridia enzymes), engineered cells comprising the vectors, and methods of using biosynthetic enzymes from gut microbiome-derived bacterium (e.g., Clostridia enzymes) to produce fatty acid amides.
Claims20
1-38. (canceled)
A vector for the production of fatty acid amides, comprising
- genes, wherein each of the genes is under the control of a regulatory element, wherein each of the genes is derived from a gut microbiome-derived bacterium, and wherein the genes encode at least a fatty acyl transferase, an acyl carrier protein, and a fatty acyl-ACP synthetase.
55-74. (canceled)
A method of producing fatty acid amides, the method comprising:
- contacting a composition, comprising one or more exogenous fatty acids and one or more amines, with a set of biosynthetic enzymes which are human gut microbiome-derived clostridia biosynthetic enzymes in an effective amount to produce a fatty acid amide, wherein the set of biosynthetic enzymes comprise a fatty acyl transferase, an acyl carrier protein, and a fatty acyl-ACP synthetase.
Dependencies are inferred from the claim text.