Metabolic engineering of cupriavidus necator for improved formate utilization
AssigneeAlliance for Sustainable Energy, LLC
InventorsChristopher Hugh CALVEY, Christopher W. JOHNSON
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Abstract
Disclosed herein are compositions and methods to improve Cupriavidus sp. as a host for formate conversion. Also disclosed herein are compositions and methods to improve growth of non-naturally occurring Cupriavidus sp. on carbon dioxide, succinate, formate and fructose as sole carbon sources.
Claims23
A non-naturally occurring Cupriavidus sp. comprising at least one genetic deletion wherein the at least one genetic deletion improves growth on formate as a sole carbon source by up to 24 percent over a naturally occurring Cupriavidus sp.
A non-naturally occurring Cupriavidus sp. comprising at least one genetic deletion wherein the at least one genetic deletion improves growth on fructose as a sole carbon source by up to 19 percent over a naturally occurring Cupriavidus sp.
A non-naturally occurring Cupriavidus sp. comprising at least one genetic deletion wherein the at least one genetic deletion improves growth on succinate as the sole carbon source by up to 7 percent over a naturally occurring Cupriavidus sp.
A non-naturally occurring Cupriavidus sp. comprising at least one genetic deletion wherein the at least one genetic deletion improves growth on carbon dioxide as a sole carbon source when compared to a naturally occurring Cupriavidus sp.
A method for deleting a megaplasmid within an organism comprising deleting a gene on the megaplasmid that encodes for a toxin;
- and further comprising deleting a replication region of the megaplasmid.
Dependencies are inferred from the claim text.