Fibroblast mediated expansion and augmentation of immune regulatory cells for treatment of acute respiratory distress syndrome (ards)
AssigneeFigene, LLC
InventorsThomas ICHIM, Pete O'HEERON
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Abstract
Embodiments of the disclosure encompass methods and compositions related to treatment of Acute Respiratory Distress Syndrome caused by any reason, including caused by a coronavirus, for example. In particular embodiments, fibroblasts are delivered to an individual in need thereof to stimulate generation of T regulatory cells that may or may not be FoxP3-positive, and/or immune regulatory cells previously exposed to fibroblasts are delivered to the individual.
Claims43
A method of preventing or treating Acute Respiratory Distress Syndrome (ARDS) in an individual, comprising administering to the individual an effective amount of:
- (a) fibroblasts and/or fibroblast-derived exosomes under conditions to allow for said fibroblasts to stimulate generation of T regulatory cells in vivo;
- and/or
- (b) immune regulatory cells, wherein said immune regulatory cells have been exposed ex vivo or in vitro under suitable conditions to fibroblasts and/or fibroblast-derived exosomes.
A method for generating a T cell population capable of suppressing pulmonary edema, wherein said T cell population comprises CD4+CD25+ regulatory T cells that are generated from freshly isolated CD4+CD25− T cells, the method comprising:
- isolating CD4+CD25− T cells from a sample comprising T cells obtained from a mammalian individual;
- contacting the isolated CD4+CD25− T cells in a culture vessel with one or more CD4+CD25+ induction agents for a time period sufficient to generate CD4+CD25+ regulatory T cells;
- and selecting the CD4+CD25+ cells.
A method of treating an inflammatory condition in an individual comprising administering to the individual a therapeutically effective amount of antigen-presenting cells and a therapeutically effective amount of one or both of fibroblasts and mesenchymal stem cells (MSCs).
Dependencies are inferred from the claim text.