Non-human animal secretome models
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsJie Liu, Toren Finkel
Life of this patent
Estimating the term…
Abstract
This document relates to methods and materials involved in the deconvolution of serum. For example, transgenic non-human animals (e g , transgenic mice) that secrete tagged (e.g., biotinylated) molecules from a particular tissue are provided.
Claims28
A transgenic non-human animal, wherein the somatic cells of said non-human animal comprise nucleic acid comprising a nucleic acid sequence encoding a polypeptide comprising biotin ligase activity and an endoplasmic reticulum (ER) retention signal.
7-9. (canceled)
16-17. (canceled)
22-23. (canceled)
A method for making a transgenic non-human animal, wherein said method comprises mating a first mating partner with a second mating partner of the opposite sex, as compared to said first mating partner and of the same species as said first mating partner, to produce at least one offspring, wherein said first mating partner is a first transgenic non-human animal, wherein the somatic cells and gametes of said first transgenic non-human animal comprise a first nucleic acid comprising a nucleic acid sequence encoding a polypeptide comprising biotin ligase activity and an endoplasmic reticulum (ER) retention signal, wherein said second mating partner is a second transgenic non-human animal, wherein the somatic cells and gametes of said second transgenic non-human animal comprise a second nucleic acid comprises a nucleic acid sequence encoding a polypeptide comprising recombinase activity, and wherein the gamete of said first mating partner that results in said offspring comprises said first nucleic acid.
28-34. (canceled)
A transgenic non-human animal, wherein the somatic cells of a cell type of said non-human animal comprise nucleic acid comprising a promoter sequence operably linked to a nucleic acid sequence encoding a polypeptide comprising biotin ligase activity and an endoplasmic reticulum (ER) retention signal, wherein said somatic cells of said cell type express said polypeptide.
36-56. (canceled)
58-65. (canceled)
Dependencies are inferred from the claim text.