Electrochemical measuring of reactive oxygen species (ros) levels in peripheral blood to detect ratio of low-density neutrophils (ldns) to high-density neutrophils (hdns), suitable to alarm presence of cancer in suspicious cases

A system for real-time detecting cancer by analyzing unprocessed blood, comprising:

• a sensor, comprising:
• a substrate;
• and
• three electrodes formed on the substrate, the three electrodes comprising a working electrode, a counter electrode, and a reference electrode, each respective electrode comprising:
• an electrically conductive layer deposited on the substrate, the electrically conductive layer comprising a proximal end and a circular distal end;
• and
• an array of multi-walled carbon nanotubes (VAMWCNTs) grown on the circular distal end, the array of VAMWCNTs configured to be put in contact with an unprocessed blood sample dropped on surface of the sensor, the unprocessed blood sample being drawn from a suspected person to have cancer;
• an electrical stimulator-analyzer device electrically connected to the sensor at the respective proximal end of each respective electrode of the three electrodes, the stimulator-analyzer device configured to:
• apply a set of voltages in a sweeping range from −0.8 V to +0.8 V to the sensor comprising the VAMWCNTs being in contact with the unprocessed blood sample;
• and
• measure a produced set of electrical currents of the sensor responsive to the applied set of voltages;
• and
• a processing unit electrically connected to the electrical stimulator-analyzer device, the processing unit comprising:
• a memory having processor-readable instructions stored therein;
• and
• a processor configured to access the memory and execute the processor-readable instructions, which, when executed by the processor configures the processor to perform a method, the method comprising:
• applying, utilizing the stimulator-analyzer device, the set of voltages in the sweeping range from −0.8 V to +0.8 V between the reference electrode and the working electrode;
• measuring, utilizing the stimulator-analyzer device, the produced set of electrical currents between the counter electrode and the working electrode;
• measuring a level of a ratio of low-density neutrophils (LDNs) to high-density neutrophils (HDNs) in the unprocessed blood sample by measuring a level of reactive oxygen species (ROS) in the unprocessed blood sample, measuring the level of ROS in the unprocessed blood sample comprising measuring a maximum electrical current of the measured set of electrical currents;
• and
• detecting cancer status of the suspected person, comprising:
• detecting a cancer disease in suspected person's body if the measured ratio of LDNs to HDNs in the unprocessed blood sample is more than 1 by detecting the measured maximum electrical current of the measured set of electrical currents being less than a first threshold electrical current value;
• or
• detecting no cancer disease in the suspected person's body if the measured ratio of LDNs to HDNs in the unprocessed blood sample is less than 1 by detecting the measured maximum electrical current of the measured set of electrical currents being more than a second threshold electrical current value.