Compositions and methods for producing disease suppression
AssigneesTu Biomics, Inc., Board of Regents of the Nevada System of Higher Education, on behalf of the Desert Research Institute
InventorsJohn Albert ARNONE, III, Richard Louis JASONI, David Charles VUONO, Brian SPEICHER
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Abstract
Provided herein are compositions, systems, and methods for treating plants, soil, fungal, and/or pathogens. More specifically, the present disclosure relates to compositions having one or more microbial metabolites from a microbial cell bath mixture for the treatment of harmful plant, agriculture, and/or soil pathogens, and also relates to methods of making and using the compositions.
Claims34
A method for preparing a biocontrol agent, the method comprising:
- a) aerobically incubating a mixture comprising a soil microbiome and a solution for at least about 1-3 days;
- b) anaerobically incubating the mixture for at least about 1-3 days;
- and
- c) removing solids from the mixture and retaining a conditioned media comprising soil microbiome metabolites, thereby preparing a biocontrol agent.
A method for preparing a biocontrol agent, the method comprising:
- a) aerobically incubating a mixture comprising a soil microbiome in solution, the soil microbiome comprising two or more bacteria selected from the group consisting of Acidobacteria, Actinobacteria, Bacteroidetes, Chlamydiae, Chloroflexi, Cyanobacteria, Deinococcus-Thermus, Firmicutes, Gemmatimonadetes, Hydrogenedentes, Nitrospirae, Parcubacteria, Planctomycetes, Proteobacteria, Saccharibacteria, Spirochaetes, Tenericutes, Thaumarchaeota, and Verrucomicrobia;
- b) anaerobically incubating the soil microbiome in solution for at least about 1-3 days;
- and
- c) removing microbial cells from the mixture and retaining a conditioned media comprising soil microbiome metabolites, thereby preparing a biocontrol agent.
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A liquid biocontrol agent comprising metabolites of a soil microbiome, wherein the soil microbiome comprises two or more bacteria selected from the group consisting of Acidobacteria, Actinobacteria, Bacteroidetes, Chlamydiae, Chloroflexi, Cyanobacteria, Deinococcus-Thermus, Firmicutes, Gemmatimonadetes, Hydrogenedentes, Nitrospirae, Parcubacteria, Planctomycetes, Proteobacteria, Saccharibacteria, Spirochaetes, Tenericutes, Thaumarchaeota, and Verrucomicrobia, wherein the liquid biocontrol agent has anti-fungal activity.
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A method of controlling a fungal pathogen, the method comprising contacting a soil or plant comprising the fungal pathogen with a biocontrol agent comprising metabolites of a soil microbiome, wherein the soil microbiome comprises two or more bacteria selected from the group consisting of Acidobacteria, Actinobacteria, Bacteroidetes, Chlamydiae, Chloroflexi, Cyanobacteria, Deinococcus-Thermus, Firmicutes, Gemmatimonadetes, Hydrogenedentes, Nitrospirae, Parcubacteria, Planctomycetes, Proteobacteria, Saccharibacteria, Spirochaetes, Tenericutes, Thaumarchaeota, and Verrucomicrobia.
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Dependencies are inferred from the claim text.