Reduced leaching of a ligand
OwnerSteven JOSEPHS, Amir JAFRI +1inventors
InventorsSteven JOSEPHS, Amir JAFRI, Mark RAPTIS
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Abstract
A column for removal of a component from a fluid is disclosed. The column has a compartment with a cross sectional area. The compartment contains beads having a diameter. A ligand selected to bind to the component is coupled to the beads. The cross-sectional area and bead diameter are selected to maintain a flow velocity of the fluid within the compartment below a first threshold, thereby reducing leaching of the ligand into the fluid. Also described herein is an adsorbent comprising a ligand that is attached to a substrate by an amine bond, wherein the ligand is resistant to dissociation from the substrate.
Claims25
A column for removal of a component from a fluid, the column comprising:
- a compartment comprising a cross-sectional area;
- a bead having a diameter and disposed within the compartment;
- and
- a ligand coupled to the bead and selected to bind to the component;
- wherein the cross-sectional area and bead diameter are selected to maintain a flow velocity of the fluid within the compartment below a first threshold.
A ligand for removal of a component from a fluid, the ligand comprising:
- at least two monomers each comprising a site that will couple to the component;
- a first linker coupled by chemical bonds between two of the monomers;
- and
- a second linker coupled by chemical bonds between one of the monomers and a substrate.
A column for use in removing a component from a fluid, the column comprising:
- a compartment;
- a bead disposed within the compartment, the bead comprising:
- a substrate;
- and
- a ligand coupled to the substrate, the ligand comprising:
- at least two monomers each comprising a site that will couple to the component;
- a first linker coupled by chemical bonds between two of the monomers;
- and
- a second linker coupled by chemical bonds between one of the monomers and the substrate.
A method of preparing an apheresis particle, comprising the steps of:
- oxidizing a substrate;
- forming a Schiff base between a ligand comprising a portion of Tumor Necrosis Factor alpha (TNF-alpha) and the oxidized substrate;
- and
- converting the Schiff base to a secondary amine bond.
Dependencies are inferred from the claim text.