Methods of enumerating particles present in a cell composition
AssigneeJuno Therapeutics, Inc.
InventorsKien KHUU-DUONG, Calvin CHAN, Rachel K. YOST, Brian CHRISTIN, Ruth BERRY, Janelle STOOPS
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Abstract
Provided herein are methods of assessing or determining the presence or absence of particles, such as bead particles, present in a cell composition. Also provided are articles of manufacture and kits for use in the methods.
Claims89
A method for enumerating or detecting the presence or absence of particles in a cell composition, the method comprising the steps of:
- a) performing one or more incubations, thereby producing an output composition, wherein the one or more incubations comprise incubating a sample comprising at least a portion of a cell composition or a sample derived from the cell composition under a condition sufficient to induce osmotic lysis of one or more cells in the sample;
- and
- b) determining the presence, absence, number and/or concentration of particles in the output composition, thereby enumerating or detecting the presence or absence of particles in the cell composition.
A method for enumerating or detecting the presence or absence of particles in a cell composition, the method comprising the steps of:
- a) performing one or more incubations, thereby producing an output composition, wherein the one or more incubations in step a) comprises:
- i) incubating a sample comprising at least a portion of a cell composition or a sample derived from the cell composition with a hypotonic solution or a hypertonic solution under a condition sufficient to induce lysis of one or more cells in the sample, thereby producing a lysed cell composition, and
- ii) incubating at least a portion of the lysed cell composition or a sample derived from the lysed cell composition with the other of the hypotonic solution or the hypertonic solution;
- and
- b) determining the presence, absence, number and/or concentration of particles in the output composition, thereby enumerating or detecting the presence or absence of particles in the cell composition.
A method of enumerating or detecting the presence or absence of particles in a cell composition, the method comprising the steps of:
- a) performing one or more incubations, thereby producing an output composition, wherein the one or more incubations in step a) comprises
- i) incubating a sample comprising at least a portion of a cell composition or a sample derived from the cell composition with a hypotonic solution under a condition sufficient to induce lysis of one or more cells in the sample, thereby producing a lysed cell composition, and
- ii) incubating at least a portion of the lysed cell composition or a sample derived from the lysed cell composition with a hypertonic solution;
- and
- b) determining the presence, absence, number and/or concentration of particles in the output composition, thereby enumerating or detecting the presence or absence of particles in the cell composition.
A method of enumerating or detecting the presence or absence of particles in a cell composition, the method comprising the steps of:
- a) performing one or more incubations, thereby producing an output composition, wherein the one or more incubations comprise incubating a sample comprising at least a portion of a cell composition or a sample derived from the cell composition, under a condition sufficient to induce lysis of one or more cells in the sample;
- and
- b) determining the presence, absence, number and/or concentration of particles in the output composition using a binding agent that specifically binds to a material, moiety or biomolecule present on, associated with or attached to the particle, thereby enumerating or detecting the presence or absence of particles in the cell composition.
An article of manufacture, comprising:
- a container comprising a solution for effecting osmotic cell lysis;
- packaging material;
- and
- a label or package insert comprising instructions for enumerating or detecting the presence or absence of particles in a cell composition.
Dependencies are inferred from the claim text.