Bacterial consortium comprising at least one bacillus and lactobacillus strain for gluten degradation
AssigneeEvonik Operations GmbH
InventorsBodo SPECKMANN, Michael SCHWARM, Stefan PELZER, Thomas BERNGRUBER, Marco GOBBETTI, Raffaella DI CAGNO
Life of this patent
Estimating the term…
Abstract
A preparation comprising probiotic strains belonging to the genera Bacillus sp., Lactobacillus sp., optionally Pediococcus sp. as viable cells or cytoplasmic extract thereof, and proteases, wherein the strains can degrade peptide sequences of gliadins. The preparation can be used to produce gluten-free foods from gluten-containing cereals or to treat gluten-related disorders.
Claims17
A preparation comprising a consortia of at least one bacterial strain selected from the genus Bacillus and at least one bacterial strain selected from the genus Lactobacillus, for use in the degradation of gluten to a gluten content of 20 ppm or less,
- wherein the consortia of strains can degrade the 12-mer peptide QLQPFPQPQLPY (Seq-ID No 1), the 14-mer peptide PQPQLPYPQPQSFP (Seq-ID No 2), the 20-mer peptide QQLPQPQQPQQSFPQQQRPF (Seq-ID No 3), and the 33-mer peptide LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF (Seq-ID No 4).
A preparation, comprising at least one strain of each of the following groups 1-5:
- Group 1:
- Lactobacillus paracasei (Lacticaseibacillus paracasei) DSM 33373, Lactobacillus paracasei (Lacticaseibacillus paracasei) DSM 33375,
- Group 2:
- Bacillus subtilis DSM 33298, Bacillus pumilus DSM 33297, Bacillus licheniformis DSM 33354, Bacillus megaterium DSM 33356, Pediococcus pentosaceus DSM 33371, Lactobacillus plantarum (Lactiplantibacillus plantarum) DSM 33370, Lactobacillus brevis (Levilactobacillus brevis) DSM 33377, Lactobacillus paracasei (Lacticaseibacillus paracasei) DSM 33376, Lactobacillus paracasei (Lacticaseibacillus paracasei) DSM 33375, Lactobacillus reuteri (Limosilactobacillus reuteri) DSM 33374, Lactobacillus plantarum (Lactiplantibacillus plantarum) DSM 33367, Lactobacillus plantarum (Lactiplantibacillus plantarum) DSM 33363, Lactobacillus paracasei (Lacticaseibacillus paracasei) DSM 33373, Lactobacillus plantarum (Lactiplantibacillus plantarum) DSM 33362,
- Group 3:
- Lactobacillus sanfranciscensis (Fructilactobacillus sanfranciscensis) DSM 33378, Lactobacillus sanfranciscensis (Fructilactobacillus sanfranciscensis) DSM 33379, Pediococcus pentosaceus DSM 33371, Lactobacillus plantarum (Lactiplantibacillus plantarum) DSM 33370, Lactobacillus plantarum (Lactiplantibacillus plantarum) DSM 33369, Lactobacillus reuteri (Limosilactobacillus reuteri) DSM 33374, Lactobacillus plantarum (Lactiplantibacillus plantarum) DSM 33363, Lactobacillus paracasei (Lacticaseibacillus paracasei) DSM 33373,
- Group 4:
- Bacillus subtilis DSM 33353, Bacillus pumilus DSM 33355, Bacillus pumilus DSM 33301,
- Group 5:
- Bacillus megaterium DSM 33300, Lactobacillus sanfranciscensis (Fructilactobacillus sanfranciscensis) DSM 33378, Pediococcus pentosaceus DSM 33371, Lactobacillus brevis (Levilactobacillus brevis) DSM 33377, Lactobacillus plantarum (Lactiplantibacillus plantarum) DSM 33368, Lactobacillus reuteri (Limosilactobacillus reuteri) DSM 33374, Lactobacillus plantarum (Lactiplantibacillus plantarum) DSM 33367, Lactobacillus plantarum (Lactiplantibacillus plantarum) DSM 33366, Lactobacillus plantarum (Lactiplantibacillus plantarum) DSM 33364, and Lactobacillus paracasei (Lacticaseibacillus paracasei) DSM 33373.
Dependencies are inferred from the claim text.