Metabolic engineering for production of lipoic acid
AssigneeNational University of Singapore
InventorsMatthew Wook Chang, Binbin Chen, Jee Loon Foo, Hua Ling
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Abstract
The present invention provides for a method to increase the free lipoic acid production in an isolated genetically engineered bacteria or yeast cell. The method involves culturing in a cysteine supplemented culture medium the engineered bacteria or yeast that is transformed with a recombinant expression vector encoding polynucleotide molecules that results in the overexpression of the following genes that are linked to at least one promoter: (1) substrate protein (e.g. Gcv3p); (2) octanoyltransferase or lipoyl synthase; (3) cofactor S-adenosyl methionine synthase; and (4) lipoamidase. The invention also relates to the engineered bacteria or yeast cell thereof.
Claims21
An isolated genetically engineered bacteria or yeast cell,
- wherein the cell has been transformed by at least one polynucleotide molecule;
- the at least one polynucleotide molecule comprising lipoic acid pathway genes which encode an octanoyltransferase, a lipoyl synthase, a protein substrate that is lipoylated, a lipoamidase and/or an S-adenosylmethionine synthase, operably linked to at least one promoter,
- wherein at least one lipoic acid pathway gene is heterologous and said genetically engineered bacteria or yeast cell is capable of increased production of free lipoic acid compared to a non-transformed cell.
Dependencies are inferred from the claim text.