Method of delivering nucleic acid to immune cells using rbcev
AssigneesNational University of Singapore, Cornell University
InventorsMinh Thi Nguyet Le, Tien Luyen Vu, Andrew Grimson, Brian Rudd, Kristel Joy Yee Mon, Tuan Thach Pham, Boya Peng
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Abstract
The invention relates to the use of red blood cells extracellular vesicle (RBCEV) to deliver nucleic acids to immune cells (e.g. T cells), wherein the nucleic acid cargoes are loaded into the RBCEV in vitro or ex vivo. The invention also relates to the prophylactic and therapeutic use of said immune cells in diseases, such as cancer.
Claims19
A method for delivering a nucleic acid into an immune cell, the method comprising incubating the immune cell with a red blood cell extracellular vesicle (RBCEV) loaded with a nucleic acid cargo.
A method of transducing an immune cell with a nucleic acid, the method comprising incubating the immune cell with a RBCEV loaded with a nucleic acid cargo.
Use of a RBCEV loaded with a nucleic acid cargo for delivering the nucleic acid into an immune cell.
An immune cell comprising an exogenous nucleic acid, wherein the nucleic acid is or has been delivered using a RBCEV.
A composition comprising one or more immune cells comprising an exogenous nucleic acid, wherein the nucleic acid is or has been delivered using a RBCEV.
Dependencies are inferred from the claim text.