Back to results
US 2023/0120734 A1Published application
Lateral flow assay device for detection of analytes and method of detection thereof
AssigneeNeuome Peptides Pte. Ltd.
InventorRajanikanth Vangala
Life of this patent
Estimating the term…
Abstract
A lateral flow assay device for detection of an analyte in a sample and a method of detection thereof is provided. A quantitative assay for detection of an analyte in a sample is provided. A conjugate is provided. A method of diagnosing COVID 19 in a patient is provided.
Claims20
A lateral flow assay device comprising:
- a porous membrane configured to be mounted on an assay support, wherein the porous membrane includes,
- a sample pad for receiving a liquid sample of a target analyte at a first end, and
- an absorbent pad at a second end, wherein the porous membrane is configured to allow capillary flow of the liquid sample from the sample pad to the absorbent pad,
- a conjugate pad including a gold nanoparticle sensor conjugate having gold nanoparticles of about 10 nm to about 20 nm conjugated with at least one of, a peptide that specifically binds with a protein in the target analyte, and an antibody against the protein in the target analyte, and
- a test region including an immobilized capture molecule that is a peptide capable of specifically binding to at least one of, the protein in the target analyte, and the antibody against the protein in the target analyte.
2 more elements
A method for quantitatively detecting a target analyte in a sample, the method comprising:
- measuring 525 nm light absorbance of gold nanoparticles of about 10 nm to about 20 nm conjugated with at least one of, a peptide that specifically binds with a protein in the target analyte, and an antibody against the protein in the target analyte;
- mixing about 50-200 μl of a sample with about 50-100 μl the gold nanoparticles;
- measuring 700 nm light absorbance of the mixed gold nanoparticles;
- and
- calculating an absorbance ratio of the measured 525 nm to 700 nm light absorbances, wherein the absorbance ratio is inversely proportional to an amount of the target analyte in the sample.
2 more elements
Dependencies are inferred from the claim text.