Method for differentiating pluripotent stem cells into underlying connective tissue fibroblasts of an epithelium
AssigneesAdhara, Centre d'Etude des Cellules Souches, Universite D'Evry Val D'Essonne
InventorsGilles LEMAITRE, Christine BALDESCHI, Sophie DOMINGUES
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Abstract
The invention relates to a method for differentiating human pluripotent stem cells into fibroblasts, characterized in that the human pluripotent stem cells are cultured on an adherent system in the presence of a medium that is suitable for culturing fibroblasts and in the absence of feeder cells.
Claims18
A method for differentiating human pluripotent stem cells into fibroblasts, comprising the step of culturing the human pluripotent stem cells on an adherent system in the presence of a medium that is suitable for culturing fibroblasts and in the absence of feeder cells.
A method for differentiating human pluripotent stem cells into fibroblasts comprising the steps of:
- (a) optionally, forming and culturing aggregates or clusters of said human pluripotent stem cells on an adherent system to support cell attachment and growth in the presence of a medium that is suitable for culturing human pluripotent stem cells;
- (b) culturing the human pluripotent stem cells or adherent aggregates or clusters of said human pluripotent stem cells on a cell culture surface coated with a defined protein matrix coating in the presence of a medium that is suitable for culturing fibroblasts;
- (c) differentiating the human pluripotent stem cells into fibroblasts by culturing on a protein matrix in the presence of a medium that is suitable for culturing fibroblasts, for 12 to 16 days;
- d) optionally, maturing the fibroblasts obtained in step c) at least during passaging on a protein matrix in the presence of a medium that is suitable for maturation;
- e) optionally, maturing the fibroblasts obtained in step d) during passaging in the presence of a medium that is suitable for maturation, with or without the protein matrix;
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- f) selecting cells obtained at step (c), (d) or (e) to obtain a homogeneous population of fibroblasts.
Dependencies are inferred from the claim text.