Glycosyltransferases, polynucleotides encoding these and methods of use
AssigneeThe New Zealand Institute for Plant and Food Research Limited
InventorsYule WANG, Ross Graham ATKINSON, Yar-Khing YAUK, Pengmin LI
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Abstract
The invention provides a method of producing a host cell, plant cell or plant with increased trilobatin content or increased N 4′-O-glycosyltransferase activity, the method comprising transformation of the host cell or plant cell with a polynucleotide encoding a polypeptide with 4′-O-glycosyltransferase activity. The invention also provides host cells, plant cells and plants, genetically modified to contain and or express the polynucleotides.
Claims41
A method of producing a plant cell or plant with increased trilobatin content, the method comprising transformation of a plant cell with a polynucleotide encoding a polypeptide with the amino acid sequence of any one of SEQ ID NO:
- 1 to 9, or a variant of the polypeptide.
A method of producing a plant cell or plant with increased 4′-O-glycosyltransferase activity, the method comprising transformation of a plant cell with a polynucleotide encoding a polypeptide with the amino acid sequence of any one of SEQ ID NO:
- 1 to 9, or a variant of the polypeptide.
A method of producing a plant cell or plant with increased trilobatin content, the method comprising transformation of a plant cell with a polynucleotide comprising a nucleotide sequence selected from any one of the sequences SEQ ID NO:
- 10 to 18, or a variant thereof.
A method of producing a plant cell or plant with increased 4′-O-glycosyltransferase activity, the method comprising transformation of a plant cell with a polynucleotide comprising a nucleotide sequence selected from any one of the sequences SEQ ID NO:
- 10 to 18, or a variant thereof.
A method of producing a plant cell or plant with increased trilobatin content or increased 4′-O-glycosyltransferase activity, the method comprising upregulating in the plant cell or plant expression of a polypeptide with the amino acid sequence of any one of SEQ ID NO:
- 1 to 9, or a variant of the polypeptide.
A method of producing a plant cell or plant with increased trilobatin content or increased 4′-O-glycosyltransferase activity, the method comprising upregulating in the plant cell or plant expression of a polynucleotide comprising a nucleotide sequence selected from any one of the sequences SEQ ID NO:
- 10 to 18, or a variant thereof.
A genetic construct comprising a polynucleotide encoding a polypeptide with the amino acid sequence of any one of SEQ ID NO:
- 1 to 9 or a variant of the polypeptide.
A genetic construct comprising a polynucleotide comprising a nucleotide sequence selected from any one of the sequences SEQ ID NO:
- 10 to 18 or a variant thereof.
A host cell genetically modified to express a polynucleotide encoding a polypeptide with the amino acid sequence of any one of SEQ ID NO:
- 1 to 9 or a variant of the polypeptide.
A host cell genetically modified to express a polynucleotide comprising a nucleotide sequence selected from any one of the sequences SEQ ID NO:
- 10 to 18 or a variant thereof.
A plant cell genetically modified to express a polynucleotide encoding a polypeptide with the amino acid sequence of any one of SEQ ID NO:
- 1 to 9 or a variant of the polypeptide.
A plant cell genetically modified to express a polynucleotide comprising a nucleotide sequence selected from any one of the sequences SEQ ID NO:
- 10 to 18 or a variant thereof.
A method for selecting a plant with altered 4′-O-glycosyltransferase activity, the method comprising testing a plant for altered expression of a polynucleotide encoding a polypeptide with the amino acid sequence of any one of SEQ ID NO:
- 1 to 9 or a variant of the polypeptide.
A method for selecting a plant with altered 4′-O-glycosyltransferase activity, the method comprising testing a plant for altered expression of a polynucleotide comprising a nucleotide sequence selected from any one of the sequences SEQ ID NO:
- 10 to 18 or a variant thereof.
A method for selecting a plant with altered trilobatin content, the method comprising testing a plant for altered expression of a polynucleotide encoding a polypeptide with the amino acid sequence of any one of SEQ ID NO:
- 1 to 9 or a variant of the polypeptide.
A method for selecting a plant with altered trilobatin content, the method comprising testing a plant for altered expression of a polynucleotide comprising a nucleotide sequence selected from any one of the sequences SEQ ID NO:
- 10 to 18 or a variant thereof.
A method of producing trilobatin, the method comprising contacting phloretin with UDP-glucose and the expression product of an expression construct encoding a polypeptide with the amino acid sequence of any one of SEQ ID:
- NO 1 to 9 or a variant of the polypeptide, or a polynucleotide comprising a nucleotide sequence selected from any one of the sequences SEQ ID NO:
- 10 to 18 or a variant thereof, to obtain trilobatin.
Dependencies are inferred from the claim text.