US 2023/0105342 A1Published application

Method for producing recombinant proteins in insects

AssigneeFuture Fields Cellular Agriculture and Research LTD.

InventorMatthew Anderson-Baron

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Abstract

One variation of a method includes: during an initial period, modifying a population of insects to produce a target compound responsive to application of a stressor; during a growth period succeeding the initial period, cultivating the population of insects according to a set of growth conditions; and, during a treatment period succeeding the growth period, applying a dosage of the stressor to the population of insects to trigger production of the target compound. The method further includes, during a harvest period succeeding the treatment period: harvesting the population of insects; homogenizing the population of insects to form a blend including a set of secondary components and an amount of the target compound; extracting the amount of the target compound from the blend; and mixing the first amount of the first target compound with a set of stabilizing agents configured to stabilize the target compound.

Claims20

Dependent claims

A method comprising:

during an initial period, genetically modifying a population of Drosophila to produce a first target compound responsive to exposure to a heat stressor, a genome of the population of Drosophila comprising:
a first promoter sequence configured to activate responsive to exposure to the heat stressor;
and
a first target sequence linked to the first promoter sequence and corresponding to the first target compound;
during a growth period succeeding the initial period, cultivating the population of Drosophila for a first duration under a first set of growth conditions;
during a treatment period succeeding the growth period:
during a first treatment cycle within the treatment period, applying a first dosage of the heat stressor to the population of Drosophila to trigger production of the first target compound in the population of Drosophila at a first rate proportional the first dosage;
during a second treatment cycle succeeding the first treatment cycle within the treatment period, applying a second dosage of the heat stressor, the second dosage greater than the first dosage, to the population of Drosophila to trigger production of the first target compound at a second rate greater than the first rate and proportional the second dosage;
and
based on a first predicted amount of the first target compound produced in the population of Drosophila during the treatment period, harvesting the population of Drosophila;
and
during a purification period succeeding the treatment period, in response to harvesting the population of Drosophila:
homogenizing the population of Drosophila to generate a blend comprising secondary components and a first amount of the first target compound;
and
extracting the first amount of the first target compound from the blend.

12 more elements

A method comprising:

during an initial period, modifying a population of Drosophila to produce a first target compound, in a set of target compounds, a genome of the population of Drosophila comprising:
a promoter sequence inducible by a first stressor and associated with cell tissue of a first tissue type;
and
a first target sequence coupled to the first promoter sequence and corresponding to the first target compound;
during a growth period succeeding the initial period, cultivating the population of Drosophila according to a set of growth conditions;
during a treatment period succeeding the growth period, applying a first dosage of the first stressor to the population of Drosophila to activate the first promoter sequence and trigger production of the first target compound in tissue of the first tissue type in the population of Drosophila;
during a harvest period succeeding the treatment period:
harvesting the population of Drosophila;
homogenizing the population of Drosophila to form a blend comprising a set of secondary components and a first amount of the first target compound;
extracting the first amount of the first target compound from the blend;
and
mixing the first amount of the first target compound with a set of stabilizing agents configured to stabilize the first target compound to generate a compound mixture;
and
during a storage period, storing the compound mixture according to a set of storage conditions defined for the first target compound.

11 more elements

A method comprising:

during a first production cycle a first population of Drosophila genetically-modified to produce a first target compound:
during a first growth period, cultivating the first population of Drosophila according to a first set of growth conditions, a first genome of the first population of Drosophila comprising:
a first promoter sequence associated with a first stressor and corresponding to a first set of target characteristics of the first target compound;
and
a first target sequence coupled to the first promoter sequence and corresponding to the first target compound;
during a first treatment period succeeding the first growth period, applying a first dosage the first stressor to the population of Drosophila to activate the first promoter sequence and trigger production of the first target compound in the first population of Drosophila;
and
during a first harvest period succeeding the first treatment period:
harvesting the first population of Drosophila;
homogenizing the first population of Drosophila to form a first blend comprising a first set of secondary components and a first amount of the first target compound;
and
extracting the first amount of the first target compound, exhibiting the first set of target characteristics, from the first blend;
and
during a second production cycle for a second population of Drosophila genetically-modified to produce a second target compound:
during a second growth period, cultivating the second population of Drosophila according to a second set of growth conditions, a second genome of the second population of Drosophila comprising:
a second promoter sequence associated with a second stressor and corresponding to a second set of target characteristics of the second target compound;
and
a second target sequence coupled to the second promoter sequence and corresponding to the second target compound;
during a second treatment period succeeding the second growth period, applying a second dosage of the second stressor to the population of Drosophila to activate the second promoter sequence and trigger production of the second target compound in the second population of Drosophila;
and
during a second harvest period succeeding the second treatment period:
harvesting the second population of Drosophila;
homogenizing the second population of Drosophila to form a second blend comprising a second set of secondary components and a second amount of the second target compound;
and
extracting the second amount of the second target compound, exhibiting the second set of target characteristics, from the second blend.

22 more elements

Dependencies are inferred from the claim text.

Facts

Application: 18/075,362Filed 5 Dec 2022
Priority: 30 Oct 2019
Published: 6 Apr 2023
Term ends: Not estimatedThe source records don’t support an estimate.

Classification15 codes

C12N 15/8509
For animal cells: for producing genetically modified animals, e.g. transgenic
A01K 67/68
Genetically modified arthropods: genetically modified insects
C07K 14/50
Growth factors; Growth regulators: fibroblast growth factor [FGF]
C07K 14/57554
Hormones: prolactin

Family

Application62/927,788Us provisional application · filed 30 Oct 2019
Application17/086,226Continuation in part · filed 30 Oct 2020
Application63/285,938Us provisional application · filed 3 Dec 2021
Application18/075,362Continuation in part
US 11,991,994 B2Related patent
US 2021/0127650 A1Published application
US 2023/0105342 A1This patent

From the US filing history. Foreign family members aren’t listed.