Lipases, compositions, methods and uses thereof
AssigneeChr. Hansen A/S
InventorsChristian Jaeckel, Hans Raj
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Abstract
The present invention relates to a wild-type, non-engineered, microbial lipolytic enzyme with a higher specificity for short-chain fatty acids than for medium to long fatty acids, specially long fatty acids. This invention further relates to a process for preparing a food product, and to the use of the wild-type, non-engineered, microbial lipolytic enzyme.
Claims21
1-15. (canceled)
A method for preparing a food product, comprising adding to a raw material or intermediate product of the food product a lipase or host cell transformed to express a DNA sequence encoding a lipase,
- wherein the lipase is selected from:
- (i) lipases comprising the amino acid sequence of any one of SEQ ID NOs. 1-11, and
- (ii) lipases comprising an amino acid sequence having an identity to any one of SEQ ID NOs. 1-11 selected from at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, and at least 99%;
- wherein the DNA sequence encoding the lipase is selected from:
- (i) DNA sequences comprising the nucleotide sequence of any one of SEQ ID NOs. 12-23, and
- (ii) DNA sequences comprising a nucleotide sequence having an identity to any one of SEQ ID NOs. 12-23 selected from at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, and at least 99%.
A food product comprising a lipase, wherein the lipase is selected from:
- (i) lipases comprising the amino acid sequence of any one of SEQ ID NOs. 1-11, and
- (ii) lipases comprising an amino acid sequence having an identity to any one of SEQ ID NOs. 1-11 selected from at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, and at least 99%;
- wherein the food product has one or more fatty acid profiles selected from:
- (a) a fatty acid profile comprising more C4-fatty acids than one or more other fatty acids selected from C6-fatty acids, C8-fatty acids, C10-fatty acids, C12-fatty acids, C14-fatty acids, C16-fatty acids, C18:1-fatty acids, C18:2-fatty acids and C18:3-fatty acids, and
- (b) a fatty acid profile comprising more C6-fatty acids than one or more other fatty acids selected from C8-fatty acids, C10-fatty acids, C12-fatty acids, C14-fatty acids, C16-fatty acids, C18:1-fatty acids, C18:2-fatty acids and C18:3-fatty acids.
An isolated lipase selected from:
- (i) lipases comprising the amino acid sequence of any one of SEQ ID NOs. 1-11, and
- (ii) lipases comprising an amino acid sequence having an identity to any one of SEQ ID NOs. 1-11 selected from at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, and at least 99%,
- wherein the lipase is expressed in Pichia pastoris and wherein the lipase has a higher specificity towards release of C4-fatty acids as compared to release of one or more of C10-fatty acids, C8-fatty acids, C6-fatty acids, C12-fatty acids, C18:2-fatty acids, C14-fatty acids, C18:0-fatty acids, C18:1-fatty acids, and C16-fatty acids, when assessed in a dairy composition comprising milk fat.
An isolated nucleic acid molecule encoding a lipase and having a DNA sequence selected from:
- (i) DNA sequences comprising the nucleotide sequence of any one of SEQ ID NOs. 12-23, and
- (ii) DNA sequences comprising a nucleotide sequence having an identity to any one of SEQ ID NOs. 12-23 selected from at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, and at least 99%,
- wherein the lipase has a higher specificity towards release of C4-fatty acids as compared to release of one or more of C10-fatty acids, C8-fatty acids, C6-fatty acids, C12-fatty acids, C18:2-fatty acids, C14-fatty acids, C18:0-fatty acids, C18:1-fatty acids, and C16-fatty acids, when assessed in a dairy composition comprising milk fat.
Dependencies are inferred from the claim text.