Microbial fermentation for the production of isoprenoid alcohols and derivatives

A genetically engineered microorganism capable of producing a product from a gaseous substrate, the microorganism comprising a nucleic acid encoding a group of exogenous enzymes comprising at least acetyl-CoA synthase and at least one of the following:

• a) a nucleic acid encoding a group of exogenous enzymes comprising i) keto-acyl-CoA thiolase (KAT1), ii) 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase, iii) methylglutaconyl-CoA hydratase (MGCH), iv) 3-methylcrotonyl-CoA carboxylase (MCCC), v) acyl-CoA reductase (ACOAR), and vi) alcohol dehydrogenase (ADH);
• b) a nucleic acid encoding a group of exogenous enzymes comprising i) KAT1, ii) HMG-CoA synthase, iii) MGCH, iv) MCCC, v) phosphotransbutyrase butyrate kinase (Ptb-buk), vi) acetaldehyde-ferredoxin oxidoreductase (AOR), and vii) (ADH);
• c) a nucleic acid encoding a group of exogenous enzymes comprising i) KAT1 or PTAr and ACKr, ii) CoA transferase A/B (CtfAB), iii) acetoacetate decarboxylase (ADC) or ADC and hydroxyisovalerate synthase (HIVS), iv) hydroxyisovalerate thioesterase (3HBZCT), v) hydroxyisopentyl-CoA hydrolyase (HPHL), vi) ACOAR, and vii) ADH;
• d) a nucleic acid encoding a group of exogenous enzymes comprising i) KAT1 or PTAr and ACKr, ii) CoA transferase A/B (CtfAB), iii) ADC or ADC and HIVS, iv) 3HBZCT, v) HPHL, vi) Ptb-buk, vii) AOR, and ADH;
• e) a nucleic acid encoding a group of exogenous enzymes comprising i) KAT1, ii) HMG-CoA synthase, iii) 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, iv) mevalonate kinase (MK), v) phosphomevalonate kinase (PMK), vi) diphosphomevalonate decarboxylase (DMD), vii) iso-pentenyl diphosphate isomerase (IDI), viii) dimethylallyl diphosphate kinase (DMPKK), and ix) dimethylallyl phosphate kinase (DMPK);
• f) a nucleic acid encoding a group of exogenous enzymes comprising i) KAT1, ii) HMG-CoA synthase, iii) 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, iv) mevalonate kinase (MK), v) phosphomevalonate decarboxylase (PMVD), vi) iso-pentenyl phosphate isomerase (IPI), and vii) prenylphosphatase (DMPase);
• g) a nucleic acid encoding a group of exogenous enzymes comprising i) thiolase, acyl-CoA acetyltransferase, or polyketide synthase, ii) β-Ketoacyl-CoA reductase or a β-hydroxyacyl-CoA dehydrogenase, iii) β-hydroxyacyl-CoA dehydratase, iv) trans-Enoyl-CoA reductase or butyryl-CoA dehydrogenase/electron transferring flavoprotein AB (Bcd-EtfAB), v) an alcohol forming acyl-CoA reductase or aldehyde forming acyl-CoA carboxylate reductase, vi) a hydrolysis enzyme or ADH, and vii) an alcohol dehydratase;
• and
• wherein the microorganism is a C1-fixing microorganism and the product is an isoprenoid alcohol.