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US 2023/0089604 A1Published application
Anti-adsorption solution
AssigneeUniversiteit Gent
InventorsBart De Spiegeleer, Frederick Verbeke, Nathalie Bracke, Evelien Wynendaele
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Abstract
The present invention is directed to an anti-adsorption solution for the analysis of peptides. Also a process for the preparation of such an anti-adsorption solution and the use of said anti-adsorption solution are described.
Claims21
1-18. (canceled)
A process for preparing an anti-adsorption solution, the process comprising:
- (i) preparing a solution comprising one or more proteins and/or peptides at a concentration from 25 ng/mL to 1 g/mL in water;
- (ii) diluting the solution of (i) to a concentration of 2.5 ng/mL to 0.1 g/mL of the one or more proteins and/or peptides by adding to the solution a 0.1% to 99% (v/v) organic solvent solution to obtain a diluted solution;
- (iii) cooling the diluted solution obtained in (ii) to obtain a cooled solution;
- (iv) separating and isolating the cooled solution obtained in (iii) from any precipitate formed to obtain a separated solution;
- and
- (v) diluting the separated solution obtained in (iv) with a solvent comprising water to obtain the anti-adsorption solution.
3 more elements
Dependencies are inferred from the claim text.