Back to results
US 2023/0085945 A1Published application
Orthogonal safety switches to eliminate genetically engineered cells
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsMatthew H. Porteus, Michael K. Cromer, Renata Martin, Jonas Fowler, Kyle Loh
Life of this patent
Estimating the term…
Abstract
Compositions and methods are provided for depletion of pluripotent cells. In one embodiment of the invention, methods are provided for depletion of pluripotent cells from a mixed population of differentiated cells and stem cells, to provide a population of cells substantially free of pluripotent stem cells.
Claims39
A genetically engineered cell comprising:
- a safety switch integrated at a first target locus in the genome where it is operably linked to the promoter of a first gene of interest without disrupting expression of the gene of interest, which gene of interest is selectively expressed in pluripotent cells;
- wherein the safety switch encodes a switch protein that is activated by a first orthologous activating agent.
Dependencies are inferred from the claim text.