3d printed micro-millifluidic bioreactors for long-term retinal organoid maintenance

A bioreactor device comprising:

• a solid substrate having a first face and a second face, the solid substrate at least partially defining a perfusion channel, a plurality of chambers, a fluidic inlet, and a fluidic outlet;
• a first sheet disposed over the first face;
• and
• a second sheet disposed over the second face, wherein the combination of the solid substrate, the first sheet and the second sheet define the perfusion channel and each chamber of the plurality of chambers, the plurality of chambers being arranged in rows of chambers in which adjacent chambers in each row are positioned on the same side of the perfusion channel with chambers on adjacent rows are positioned on opposite sides of the perfusion channel, the perfusion channel extending from the fluidic inlet and the fluidic outlet, each chamber being in fluid communication with the perfusion channel;
• and
• a plurality of mixers positioned in the perfusion channel wherein at least one mixer is disposed between adjacent chambers.