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US 2023/0075854 A1Published application
Virus-free cell cultures
AssigneeBiomarin Pharmaceutical Inc.
InventorsMichael LOCHRIE, Peter COLOSI, Barrie CARTER, Tomas CINEK
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Abstract
The invention is directed to methods of removing adventitious virus from cells and cell lines and virus-free cells and cell lines obtainable by such methods.
Claims33
A method of removing adventitious virus from cells comprising:
- (a) growing cells in cell culture at a suboptimal temperature that is higher or lower than (i) a first optimal temperature range for replication of the adventitious virus or (ii) a second optimal temperature range for replication of the cells, for at least 2 passages, and
- (b) detecting a reduction of the adventitious virus in the cell culture following growth of the cells at the temperature.
A method of removing adventitious virus from cells comprising:
- (a) growing cells in cell culture for at least five passages in the presence of one or more antiviral agents in an amount effective to remove or cure the adventitious virus from the cell culture, and
- (b) detecting a reduction of the adventitious virus in the culture following growth in the presence of the one or more antiviral agents.
Dependencies are inferred from the claim text.