Modulating tlr/nf-kb and p53 signaling pathways to enhance interspecies chimerism between evolutionaryily distant species
AssigneeBoard of Regents, The University of Texas System
InventorsJun WU, Canbin ZHENG, Yingying HU
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Abstract
Disclosed herein are chimeric blastocysts, such as chimeric blastocysts derived from a host blastocyst from a first mammalian species and having donor pluripotent stem cells from a second mammalian species, wherein the donor pluripotent stem cells have reduced expression or reduced biological activity of one or more proteins in the TLR/NF-kB signaling pathway or the p53 pathway. Methods of preparing chimeric blastocysts and methods of obtaining mammalian organs and tissues are also provided.
Claims29
A chimeric blastocyst, comprising:
- a host blastocyst from a first mammalian species;
- and
- (ii) donor pluripotent stem cells from a second mammalian species, wherein the donor pluripotent stem cells have reduced expression or reduced biological activity of one or more proteins in the TLR/NF-kB signaling pathway or the p53 pathway.
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A method of preparing a chimeric blastocyst comprising:
- injecting a host blastocyst from a first mammalian species with donor pluripotent stem cells from a second mammalian species,
- wherein the donor pluripotent stem cells have reduced expression or reduced biological activity of one or more proteins in the TLR/NF-kB signaling pathway or the p53 pathway.
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A method of obtaining a mammalian organ or tissue comprising:
- (i) injecting a host organogenesis disabled blastocyst from a first mammalian species with donor pluripotent stem cells from a second mammalian species to form a chimeric blastocyst, wherein the donor pluripotent stem cells have reduced expression or reduced biological activity of one or more proteins in the TLR/NF-kB signaling pathway or the p53 pathway;
- (ii) implanting the chimeric blastocyst into a pseudo-pregnant mammal;
- and
- (iii) obtaining a chimeric embryo, fetus, or mammal comprising the mammalian organ or tissue.
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Dependencies are inferred from the claim text.