# US 2023/0065508 A1 · Cell-extruding device including membrane module, and cell-extruding method using same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2023/0065508 A1 - Document ID: US20230065508A1 - Status: Published application - Kind: A1 - Application number: 17/759,268 - Filed: 2020-09-18 - Published: 2023-03-02 - Earliest claimed: 2020-01-22 - Assignees: Mdimune Inc. - Applicants: Mdimune Inc. - Inventors: Dongwoo HAN; Shingyu BAE; Seungwook OH; Yunbeom SIM; Seonjoo LEE - Web page: https://patentgrep.com/patent/US20230065508A1 ## CPC - C12M 33/14: {with filters, sieves or membranes} - G01N 1/405: {by adsorption or absorption} - C12M 33/04: {by injection or suction, e.g. using pipettes, syringes, needles (pipettes in general B01L3/02)} - C12M 47/06: {Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material (lysis of microorganisms C12N1/06; extracting or separating nucleic acids from biological samples C12N15/1003)} - C12M 47/10: {Separation or concentration of fermentation products (bioreactors combined with means for distillation or extraction of liquid fuel C12M43/02)} - G01N 2001/4088: {filtration} - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Provided are a cell-extruding device including a membrane module, and a cell-extruding method using the same, wherein a suspension including cells is efficiently dispersed in the membrane module, thus preventing membrane clogging and increasing the usable area of the membrane, and thereby replacing the membrane and carrying out the process are easy, and production efficiency is increased. ## Claims ### Claim 1 A cell extrusion device comprising a membrane module for extruding cells contained in a suspension which passes through the membrane module through constant pressure, wherein the membrane module comprises: a pair of supports having a through-hole through which the suspension flows; a membrane through which the cells pass and are extruded; and a dispersion disk having a plurality of micro holes except a dispersing zone having a center, wherein the membrane and the dispersion disk are located between the pair of supports, the through-holes respectively formed in the pair of supports and the center of the dispersion disk are located on the same axis, and the membrane module is closely fixed by a pair of holders. ### Claim 2 (depends on 1) The device of claim 1, wherein the membrane module has an O-ring between the dispersion disk and a support adjacent to the dispersion disk, thereby forming a predetermined space. ### Claim 3 (depends on 1) The device of claim 1, wherein the membrane module has an O-ring between the dispersion disk and the membrane, thereby forming a predetermined space. ### Claim 4 (depends on 1) The device of claim 1, wherein the membrane module has a porous membrane supporting film located on one or more surfaces of the membrane to prevent the membrane from being damaged. ### Claim 5 A cell extrusion device comprising a membrane module for extruding cells contained in a suspension which passes through the membrane module through a predetermined pressure, wherein the membrane module comprises: a pair of supports having a through-hole through which the suspension flows; a membrane through which the cells pass and are extruded; and a pair of dispersion disks having a plurality of micro holes except a dispersing zone having a center, wherein the membrane is located between the pair of dispersion disks, the membrane and the pair of dispersion disks are located between the pair of supports, the through-holes respectively formed in the pair of supports and the center of the dispersion disk are located on the same axis, and the membrane module is closely fixed by a pair of holders. ### Claim 6 (depends on 5) The device of claim 5, wherein the membrane module has an O-ring between the support and the dispersion disk, thereby forming a predetermined space. ### Claim 7 (depends on 5) The device of claim 5, wherein the membrane module has an O-ring between the membrane and the dispersion disk, thereby forming a predetermined space. ### Claim 8 (depends on 5) The device of claim 5, wherein the membrane module has a porous membrane supporting film on one or more surfaces of the membrane to prevent the membrane from being damaged. ### Claim 9 (depends on 1) The device of claim 1, wherein, in the membrane module, a syringe containing the suspension is connected to any one or more of the through-holes formed in the pair of supports. ### Claim 10 (depends on 9) The device of claim 9, wherein, in the syringe, a plunger moves by means of a syringe pump. ### Claim 11 (depends on 1) A cell extrusion method using the cell extrusion device of claim 1, comprising: connecting a syringe having a cell-containing suspension to a through-hole of a support adjacent to the dispersion disk among the through-holes of the cell extrusion device; and injecting the suspension contained in the syringe into the membrane module and discharging the suspension through the other through-hole via the dispersion disk and the membrane, the entire process of the method being repeated at least once. ### Claim 12 (depends on 5) A cell extrusion method using the cell extrusion device of claim 5, comprising: connecting a first syringe including a cell-containing suspension to a through-hole at one side of the cell extrusion device, and connecting a second syringe in which a plunger has been pressed to a through-hole at the other side of the cell extrusion device; injecting the suspension contained in the first syringe into the membrane module to allow the suspension to flow into the second syringe through the dispersion disk and the membrane; and injecting the suspension flowing into the second syringe into the membrane module again to allow the suspension to flow into the first syringe through the dispersion disk and the membrane, and the entire process of the method is repeated at least once. ### Claim 13 (depends on 11) Vesicles prepared by the cell extrusion method of claim 11. ### Claim 14 (depends on 12) Vesicles prepared by the cell extrusion method of claim 12. ### Claim 15 (depends on 5) The device of claim 5, wherein, in the membrane module, the syringe containing the suspension is connected to any one or more of the through-holes formed in the pair of supports. ### Claim 16 (depends on 15) The device of claim 15, wherein, in the syringe, a plunger moves by means of a syringe pump.