Increase of saturated fat in soybean
AssigneeCalyxt, Inc.
InventorsQiwei Shan, Zachary Demorest, James Presnail
Life of this patent
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Abstract
Materials and methods are provided for making soybean varieties that have altered oil composition as a result of one or more mutation modulating the expression of a SACPD-C gene, a FATB-1A gene, or both the SACPD-C and FATB-1A genes. For example, a soybean plant, plant part, or plant cell producing an oil that has increased saturated fatty acid content as compared to oil produced from a corresponding soybean plant, plant part, or plant cell lacking the one or more mutations is provided.
Claims20
A soybean plant, plant part, or plant cell comprising one or more mutations modulating the expression of a SACPD-C gene, a FATB-1A gene, or both the SACPD-C and FATB-1 A genes, wherein said plant, plant part, or plant cell produces oil that has increased saturated fatty acid content as compared to oil produced from a corresponding soybean plant, plant part, or plant cell lacking the one or more mutations, and wherein the one or more mutations modulating the expression of the SACPD-C gene comprise a targeted mutation induced by a rare-cutting endonuclease.
A method for generating a soybean plant comprising a mutation modulating the expression of a SACPD-C gene, a FATB-1A gene, or both the SACPD-C and FATB-1A genes, comprising:
- (a) contacting a population of soybean plant cells from a soybean plant producing an oil with a saturated fatty acid content of about 15% of total fatty acid composition with one or more nucleic acid sequences inducing:
- (i) a mutation resulting in reduced expression of the SACPD-C gene, wherein the mutation is a targeted mutation induced by a rare cutting endonuclease;
- (ii) a mutation resulting in increased expression of the FATB-1A gene;
- or
- (iii) a combination thereof;
- (b) selecting, from the population, a cell in which expression of the SACPD-C gene has been reduced, expression of the FATB-1A gene has been increased, or expression of the SACPD-C gene has been reduced and expression of the FATB-1A gene has been increased, and
- (c) regenerating the selected plant cell into a soybean plant.
A soybean oil composition, comprising a soybean oil produced by a soybean plant, plant part, or plant cell comprising one or more mutations modulating the expression of a SACPD-C gene, a FATB-1A gene, or both the SACPD-C and FATB-1A genes, wherein the soybean oil has increased saturated fatty acid content as compared to oil produced from a corresponding soybean plant, plant part, or plant cell lacking the one or more mutations and wherein the one or more mutations modulating the expression of the SACPD-C gene comprise a targeted mutation induced by a rare-cutting endonuclease.
Dependencies are inferred from the claim text.