# US 2023/0055086 A1 · Inter-alpha inhibitor proteins

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- Number: US 2023/0055086 A1
- Document ID: US20230055086A1
- Status: Published application
- Kind: A1
- Application number: 17/407,063
- Filed: 2021-08-19
- Published: 2023-02-23
- Earliest claimed: 2021-08-19
- Assignees: ProThera Biologics, Inc.
- Applicants: ProThera Biologics, Inc.
- Inventors: Yow-Pin Lim; Denice Spero
- Web page: https://patentgrep.com/patent/US20230055086A1

## CPC

- G01N 33/566: using specific carrier or receptor proteins as ligand binding reagents {where possible specific carrier or receptor proteins are classified with their target compounds}
- G01N 33/68: involving proteins, peptides or amino acids {(involving lipoproteins G01N33/92)}
- G01N 33/5306: {Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding}
- C07K 16/18: against material from animals or humans
- G01N 2333/81: Protease inhibitors
- G01N 2400/40: Glycosaminoglycans, i.e. GAG or mucopolysaccharides, e.g. chondroitin sulfate, dermatan sulfate, hyaluronic acid, heparin, heparan sulfate, and related sulfated polysaccharides
- G01N 2400/50: Lipopolysaccharides; LPS
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

Described herein are methods for quantifying IAIP levels in a sample.

## Claims

### Claim 1

A method for quantifying Inter-alpha-Inhibitor (IαI) and Pre-alpha-Inhibitor (PαI) in a sample from a subject comprising:
a) contacting the sample with a binding agent to produce an IαI or PαI binding agent complex, wherein the binding agent is bound to a support;
b) contacting the IαI or PαI binding agent complex with a detection agent; and
c) detecting an amount of the detection agent bound to the IαI or PαI binding agent complex
wherein the IαI or PαI comprises at least one heavy chain selected from the group consisting of H1, H2, and H3 chain;
wherein the binding agent comprises an antibody, and the detection agent comprises a ligand that binds to at least one heavy chain,
thereby quantifying IαI and PαI in the sample.