# US 2023/0054722 A1 · Rapeseed protein composition with high protein quality > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2023/0054722 A1 - Document ID: US20230054722A1 - Status: Published application - Kind: A1 - Application number: 17/786,247 - Filed: 2020-12-17 - Published: 2023-02-23 - Earliest claimed: 2019-12-20 - Assignees: Dsm IP Assets B.V. - Applicants: DSM IP Assets B.V. - Inventors: Arjen SEIN; Maaike Johanna BRUINS; Ines WARNKE - Web page: https://patentgrep.com/patent/US20230054722A1 ## CPC - A23L 33/185: Vegetable proteins - C07K 14/415: from plants - A23C 11/103: {containing only proteins from pulses, oilseeds or nuts, e.g. nut milk} - A23C 20/025: {mainly containing proteins from pulses or oilseeds} - A23J 1/14: from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds - A23J 3/14: Vegetable proteins - A23L 9/24: {containing non-milk fats and non-milk proteins, e.g. eggs or soybeans} - A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - A23C: DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF (preservation thereof A23B11/00) - A23J: PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS ## Abstract The present invention is directed to a rapeseed protein isolate having a DIAAS value which is equal to or higher than 50. Further, the invention relates to a method for preparing such rapeseed protein isolate, to the use of such rapeseed protein isolate and to a food product comprising a rapeseed protein isolate having a high DIAAS value. ## Claims ### Claim 1 A rapeseed protein isolate having a Digestible Indispensable Amino Acid Score or DIAAS value which is equal to or higher than 50. ### Claim 2 (depends on 1) The rapeseed protein isolate according to claim 1, having a ΔH value as determined by Differential Scanning Calorimetry of a 40% solution or dispersion of said rapeseed protein isolate in water of 0±0.5 J/g. ### Claim 3 (depends on 1) The rapeseed protein isolate according to claim 1, having a DIAAS value in older children, adolescents and adults aged 3 yr. and older which is equal to or higher than 100. ### Claim 4 (depends on 1) The rapeseed protein isolate according to claim 1 wherein said DIAAS value in older children, adolescents and adults aged 3 yr. and older is 110±10. ### Claim 5 (depends on 1) The rapeseed protein isolate according to claim 1, further having a DIAAS value in children aged 6 mo. to 3 yr. which is equal to or higher than 90, optionally 100±10. ### Claim 6 (depends on 1) The rapeseed protein isolate according to claim 1, further having a DIAAS value in infants aged 0 to 6 mo. which is equal to or higher than 50. ### Claim 7 (depends on 1) The rapeseed protein isolate according to claim 1 wherein said DIAAS value in infants aged 0 to 6 mo. is 60±10. ### Claim 8 (depends on 1) The rapeseed protein isolate according to claim 1 wherein said DIAAS value is calculated using equation DIAAS (%)=100×lowest value of the digestible indispensable amino acid reference ratio. ### Claim 9 (depends on 1) The rapeseed protein isolate according to claim 1 wherein the rapeseed protein isolate comprises from 40 to 65% (w/w) cruciferins and from 35 to 60% (w/w) napins, the total being equal to or less than 100% (w/w) or comprises 80-100% (w/w) cruciferins and 0-20% (w/w) napins, or comprises 0-20% (w/w) cruciferins and 80-100% (w/w) napins, wherein the sum of cruciferins and napins is not exceeding 100% (w/w). ### Claim 10 (depends on 1) The rapeseed protein isolate according to claim 1 having a solubility in water of from 1% to 60%, when measured at 6.8±0.1 and 20±1° C. ### Claim 11 (depends on 1) A method for preparing a rapeseed protein isolate according to claim 1, comprising incubating a mixture comprising native rapeseed protein isolate and water at a temperature of from 70 to 100° C. for a period of from 2 to 100 min or by incubating at a temperature of from 120 to 150° C. for a period of from 2 seconds to 5 minutes. ### Claim 12 (depends on 11) The method according to claim 11 further comprising homogenizing and drying. ### Claim 13 (depends on 1) A method for making a food product comprising: a) mixing a rapeseed protein isolate according to claim 1 with a food product, or b) mixing a native rapeseed protein isolate with a food product and incubating the obtained mixture at a temperature of from 70 to 100° C. for a period of from 2 to 100 min or by incubating at a temperature of from 120 to 150° C. for a period of from 2 seconds to 5 minutes. ### Claim 14 (depends on 1) A food product prepared with a rapeseed protein isolate according to claim 1. ### Claim 15 (depends on 1) A food product comprising a rapeseed protein isolate according to claim 1.