# US 2023/0054527 A1 · Enhanced disease resistance of maize to northern corn leaf blight by a qtl on chromosome 4

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- Number: US 2023/0054527 A1
- Document ID: US20230054527A1
- Status: Published application
- Kind: A1
- Application number: 17/783,997
- Filed: 2020-12-18
- Published: 2023-02-23
- Earliest claimed: 2019-12-20
- Assignees: KWS SAAT SE & Co. KGaA
- Applicants: KWS SAAT SE & Co. KGaA
- Inventors: Daniela SCHEUERMANN; Thomas PRESTERL; Bettina KESSEL; Dietmar STAHL; Daniel Fabian STIRNWEIS
- Web page: https://patentgrep.com/patent/US20230054527A1

## CPC

- C12Q 1/6895: for plants, fungi or algae
- A01H 6/4684: {Zea mays [maize]}
- C12Q 2600/13: Plant traits
- C12Q 2600/156: Polymorphic or mutational markers
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

The present invention relates to maize plants having increased pathogen resistance or tolerance, in particular increased resistance or tolerance to pathogens causing Northern Corn Leaf Blight, i.e. Exserohilum turcicum. Such maize plants can be characterized as having a particular QTL allele comprising one or more resistance gene, or particular molecular markers in chromosome 4 based on donor line H102. The invention further relates to methods for generating such maize plants, as well as methods for identifying such maize plants.

## Claims

### Claim 1

A method for identifying a maize plant or plant part, comprising screening for the presence of a QTL allele located on chromosome 4 in a maize plant or plant part, wherein said QTL allele is located on a chromosomal interval flanked by marker alleles A and B, wherein marker alleles A and B are SNPs which are respectively adenine (A) at the position 16107190 bp on reference genome B73 AGPv04 and cytosine (C) at the position 18470371 bp on reference genome B73 AGPv04.

### Claim 2 (depends on 1)

The method according to claim 1, wherein said QTL allele comprises one or more of marker alleles M1 to M56, wherein
M1 is a SNP which is thymine (T) at the position 1071 of the coding sequence of Zm00001d049121 referenced to the B73 reference genome AGPv4;
M2 is a SNP which is guanine (G) at the position 1302 of the coding sequence of Zm00001d049121 referenced to the B73 reference genome AGPv4;
M3 is a SNP which is cytosine (C) at the position 1497 of the coding sequence of Zm00001d049121 referenced to the B73 reference genome AGPv4;
M4 is a SNP which is guanine (G) at the position 1631 of the coding sequence of Zm00001d049121 referenced to the B73 reference genome AGPv4;
M5 is a SNP which is cytosine (C) at the position 1691 of the coding sequence of Zm00001d049121 referenced to the B73 reference genome AGPv4;
M6 is an indel which is deletion of the nucleotide at the position 1807 of the coding sequence of Zm00001d049121 referenced to the B73 reference genome AGPv4;
M7 is an indel which is an insertion of one or more nucleotides between nucleotides at positions 1815 and 1816 of the coding sequence of Zm00001d049121 referenced to the B73 reference genome AGPv4;
M8 is a SNP which is guanine (G) at the position 2045 of the coding sequence of Zm00001d049121 referenced to the B73 reference genome AGPv4;
M9 is an indel which is deletion of nucleotides at positions 2424-2429 of Zm00001d049121 referenced to the B73 reference genome AGPv4;
M10 is a SNP which is cytosine (C) at the position 2458 of the coding sequence of Zm00001d049121 referenced to the B73 reference genome AGPv4;
M11 is a SNP which is adenine (A) at the position 2530 of the coding sequence of Zm00001d049121 referenced to the B73 reference genome AGPv4;
M12 is a SNP which is adenine (A) at the position 2683 of the coding sequence of Zm00001d049121 referenced to the B73 reference genome AGPv4;
M13 is a SNP which is cytosine (C) at the position 12 of the coding sequence of Zm00001d049113 referenced to the B73 reference genome AGPv4;
M14 is a SNP which is cytosine (C) at the position 291 of the coding sequence of Zm00001d049113 referenced to the B73 reference genome AGPv4;
M15 is a SNP which is thymine (T) at the position 309 of the coding sequence of Zm00001d049113 referenced to the B73 reference genome AGPv4;
M16 is a SNP which is thymine (T) at the position 324 of the coding sequence of Zm00001d049113 referenced to the B73 reference genome AGPv4;
M17 is an indel which is deletion of nucleotides at positions 415-417 of the coding sequence of Zm00001d049113 referenced to the B73 reference genome AGPv4;
M18 is a SNP which is guanine (G) at the position 195 of the coding sequence of Zm00001d049145 referenced to the B73 reference genome AGPv4;
M19 is a SNP which is guanine (G) at the position 247 of the coding sequence of Zm00001d049145 referenced to the B73 reference genome AGPv4;
M20 is a SNP which is cytosine (C) at the position 248 of the coding sequence of Zm00001d049145 referenced to the B73 reference genome AGPv4;
M21 is a SNP which is thymine (T) at the position 1097 of the coding sequence of Zm00001d049145 referenced to the B73 reference genome AGPv4;
M22 is a SNP which is cytosine (C) at the position 1105 of the coding sequence of Zm00001d049145 referenced to the B73 reference genome AGPv4;
M23 is a SNP which is thymine (T) at the position 1159 of the coding sequence of Zm00001d049145 referenced to the B73 reference genome AGPv4;
M24 is a SNP which is thymine (T) at the position 1185 of the coding sequence of Zm00001d049145 referenced to the B73 reference genome AGPv4;
M25 is an indel which is an insertion of one or more nucleotides between nucleotides at positions 1216 and 1217 of the coding sequence of Zm00001d049145 referenced to the B73 reference genome AGPv4;
M26 is a SNP which is cytosine (C) at the position 1242 of the coding sequence of Zm00001d049145 referenced to the B73 reference genome AGPv4;
M27 is a SNP which is guanine (G) at the position 1282 of the coding sequence of Zm00001d049145 referenced to the B73 reference genome AGPv4;
M28 is a SNP which is guanine (G) at the position 1344 of the coding sequence of Zm00001d049145 referenced to the B73 reference genome AGPv4;
M29 is a SNP which is cytosine (C) at the position 1380 of the coding sequence of Zm00001d049145 referenced to the B73 reference genome AGPv4;
M30 is a SNP which is cytosine (C) at the position 1422 of the coding sequence of Zm00001d049145 referenced to the B73 reference genome AGPv4;
M31 is a SNP which is cytosine (C) at the position 338 of the coding sequence of Zm00001d049148 referenced to the B73 reference genome AGPv4;
M32 is a SNP which is adenine (A) at the position 412 of the coding sequence of Zm00001d049148 referenced to the B73 reference genome AGPv4;
M33 is a SNP which is thymine (T) at the position 432 of the coding sequence of Zm00001d049148 referenced to the B73 reference genome AGPv4;
M34 is a SNP which is guanine (G) at the position 447 of the coding sequence of Zm00001d049148 referenced to the B73 reference genome AGPv4;
M35 is a SNP which is thymine (T) at the position 507 of the coding sequence of Zm00001d049148 referenced to the B73 reference genome AGPv4;
M36 is a SNP which is thymine (T) at the position 509 of the coding sequence of Zm00001d049148 referenced to the B73 reference genome AGPv4;
M37 is a SNP which is thymine (T) at the position 585 of the coding sequence of Zm00001d049148 referenced to the B73 reference genome AGPv4;
M38 is a SNP which is thymine (T) at the position 588 of the coding sequence of Zm00001d049148 referenced to the B73 reference genome AGPv4;
M39 is a SNP which is adenine (A) at the position 593 of the coding sequence of Zm00001d049148 referenced to the B73 reference genome AGPv4;
M40 is a SNP which is cytosine (C) at the position 672 of the coding sequence of Zm00001d049148 referenced to the B73 reference genome AGPv4;
M41 is a SNP which is thymine (T) at the position 741 of the coding sequence of Zm00001d049148 referenced to the B73 reference genome AGPv4;
M42 is a SNP which is guanine (G) at the position 753 of the coding sequence of Zm00001d049148 referenced to the B73 reference genome AGPv4;
M43 is a SNP which is cytosine (C) at the position 846 of the coding sequence of Zm00001d049148 referenced to the B73 reference genome AGPv4;
M44 is a SNP which is thymine (T) at the position 996 of the coding sequence of Zm00001d049148 referenced to the B73 reference genome AGPv4;
M45 is a SNP which is cytosine (C) at the position 1092 of the coding sequence of Zm00001d049148 referenced to the B73 reference genome AGPv4;
M46 is a SNP which is thymine (T) at the position 1102 of the coding sequence of Zm00001d049148 referenced to the B73 reference genome AGPv4;
M47 is a SNP which is cytosine (C) at the position 1260 of the coding sequence of Zm00001d049148 referenced to the B73 reference genome AGPv4;
M48 is a SNP which is thymine (T) at the position 1300 of the coding sequence of Zm00001d049148 referenced to the B73 reference genome AGPv4;
M49 is a SNP which is cytosine (C) at the position 22 of the coding sequence of Zm00001d049159 referenced to the B73 reference genome AGPv4;
M50 is a SNP which is thymine (T) at the position 47 of the coding sequence of Zm00001d049159 referenced to the B73 reference genome AGPv4;
M51 is an indel which is an insertion of one or more nucleotides between nucleotides at positions 52 and 53 of the coding sequence of Zm00001d049159 referenced to the B73 reference genome AGPv4;
M52 is a SNP which is adenine (A) at the position 189 of the coding sequence of Zm00001d049159 referenced to the B73 reference genome AGPv4;
M53 is a SNP which is adenine (A) at the position 190 of the coding sequence of Zm00001d049159 referenced to the B73 reference genome AGPv4;
M54 is a SNP which is guanine (G) at the position 1980 of the coding sequence of Zm00001d049159 referenced to the B73 reference genome AGPv4;
M55 is a SNP which is guanine (G) at the position 2014 of the coding sequence of Zm00001d049159 referenced to the B73 reference genome AGPv4;
M56 is a SNP which is guanine (G) at the position 2160 of the coding sequence of Zm00001d049159 referenced to the B73 reference genome AGPv4;
M57 is a polymorphism, preferably a SNP, insertion or deletion, at any position of the genomic sequence of MA_H102_24892 as set forth in SEQ ID NO: 37 and/or the coding sequence of MA_H102_24892, which is suitable to detect the presence of MA_H102_24892.
preferably wherein
the B73 coding sequence of Zm00001d049121 comprises a sequence as set forth in SEQ ID NO: 11;
the B73 coding sequence of Zm00001d049113 comprises a sequence as set forth in SEQ ID NO: 13;
the B73 coding sequence of Zm00001d049145 comprises a sequence as set forth in SEQ ID NO: 15;
the B73 coding sequence of Zm00001d049148 comprises a sequence as set forth in SEQ ID NO: 17; and/or
the B73 coding sequence of Zm00001d049159 comprises a sequence as set forth in SEQ ID NO: 19.

### Claim 3 (depends on 2)

The method according to claim 2, wherein screening for the presence of said QTL allele comprises identifying any one or more of molecular markers A or B and/or identifying any one or more of marker alleles selected from M1 to M57 as defined in claim 2.

### Claim 4 (depends on 2)

A method for identifying a maize plant or plant part, comprising screening for the presence of one or more marker alleles selected from M1 to M57 as defined in claim 2.

### Claim 5 (depends on 2)

The method according to claim 2, further comprising selecting a plant or plant part comprising said QTL allele or one or more of marker alleles M1 to M57 as defined in claim 2.

### Claim 6 (depends on 1)

The method according to claim 1, wherein a plant or plant part is identified as having increased resistance to a pathogen if said QTL or one or more of said marker alleles are present.

### Claim 7 (depends on 2)

A method for generating a maize plant, comprising introducing into the genome of a plant or a plant part
a QTL allele located on chromosome 4 in a maize plant or plant part, wherein said QTL allele is located on a chromosomal interval flanked by marker alleles A and B, wherein marker alleles A and B are SNPs which are respectively adenine (A) at the position 16107190 bp on reference genome B73 AGPv04 and cytosine (C) at the position 18470371 bp on reference genome B73 AGPv04;
one or more marker allele selected from M1 to M57 as defined in claim 2;
a polynucleic acid encoding exogenous Zm00001d049121 comprising one or more, preferably all, molecular marker M1 to M12; preferably having a coding sequence as set forth in SEQ ID NO: 1 or a coding sequence having an identity of at least 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence as set forth in SEQ ID NO: 1, preferably over the entire length of the sequence; or encoding a sequence as set forth in SEQ ID NO: 2 or a sequence having an identity of at least 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence as set forth in SEQ ID NO: 2, preferably over the entire length of the sequence;
a polynucleic acid encoding exogenous Zm00001d049113 comprising one or more, preferably all, molecular marker M13 to M17; preferably having a sequence as set forth in SEQ ID NO: 3 or a coding sequence having an identity of at least 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence as set forth in SEQ ID NO: 3, preferably over the entire length of the sequence, or encoding a sequence as set forth in SEQ ID NO: 4 or a sequence having an identity of at least 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence as set forth in SEQ ID NO: 4, preferably over the entire length of the sequence;
a polynucleic acid encoding exogenous Zm00001d049145 comprising one or more, preferably all, molecular marker M18 to M30; preferably having a sequence as set forth in SEQ ID NO: 5 or a coding sequence having an identity of at least 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence as set forth in SEQ ID NO: 5, preferably over the entire length of the sequence, or encoding a sequence as set forth in SEQ ID NO: 6 or a sequence having an identity of at least 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence as set forth in SEQ ID NO: 6, preferably over the entire length of the sequence;
a polynucleic acid encoding exogenous Zm00001d049148 comprising one or more, preferably all, molecular marker M31 to M48; preferably having a sequence as set forth in SEQ ID NO: 7 or a coding sequence having an identity of at least 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence as set forth in SEQ ID NO: 7, preferably over the entire length of the sequence, or encoding a sequence as set forth in SEQ ID NO: 8 or a sequence having an identity of at least 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence as set forth in SEQ ID NO: 8, preferably over the entire length of the sequence; and/or
a polynucleic acid encoding exogenous Zm00001d049159 comprising one or more, preferably all, molecular marker M49 to M56; preferably having a sequence as set forth in SEQ ID NO: 9 or a coding sequence having an identity of at least 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence as set forth in SEQ ID NO: 9, preferably over the entire length of the sequence, or encoding a sequence as set forth in SEQ ID NO: 10 or a sequence having an identity of at least 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence as set forth in SEQ ID NO: 10, preferably over the entire length of the sequence; and/or
a polynucleic acid encoding exogenous Zm00001d049159 comprising one or more, preferably all, molecular marker M49 to M56; preferably having a sequence as set forth in SEQ ID NO: 38 or a coding sequence having an identity of at least 80%, 85%, 90%, 95%, 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence as set forth in SEQ ID NO: 38, preferably over the entire length of the sequence, or encoding a sequence as set forth in SEQ ID NO: 39 or a sequence having an identity of at least 80%, 85%, 90%, 95%, 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence as set forth in SEQ ID NO: 39, preferably over the entire length of the sequence;
preferably wherein introducing into the genome comprises mutagenesis or transgenesis.

### Claim 8 (depends on 7)

The method according to claim 7, comprising transforming a plant or plant part, preferably a plant cell or a plant tissue, more preferably a protoplast, a callus, an immature or mature embryo or an inflorescence, with a QTL allele located on chromosome 4 in a maize plant or plant part, wherein said QTL allele is located on a chromosomal interval flanked by marker alleles A and B, wherein marker alleles A and B are SNPs which are respectively adenine (A) at the position 16107190 bp on reference genome B73 AGPv04 and cytosine (C) at the position 18470371 bp on reference genome B73 AGPv04 or the polynucleic acid as defined in claim 7, and optionally regenerating a plant or plant part from said plant cell or plant tissue, preferably said protoplast, said callus, said immature or mature embryo or said inflorescence.

### Claim 9 (depends on 1)

The method according to claim 1, wherein said plant or plant part has increased resistance to a pathogen, preferably a fungal pathogen, more preferably
wherein said pathogen is an Exserohilum sp, most preferably wherein said pathogen is Exserohilum turcicum.

### Claim 10

A method for generating a maize plant, preferably a maize plant having increased pathogen resistance and/or tolerance, or a part thereof, preferably wherein said pathogen is Exserohilum turcicum, comprising
mutating endogenous Zm00001d049121 to a sequence encoding a sequence as set forth in SEQ ID NO: 2 or a sequence having an identity of at least 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence as set forth in SEQ ID NO: 2, preferably over the entire length of the sequence;
mutating endogenous Zm00001d049113 to a sequence encoding a sequence as set forth in SEQ ID NO: 4 or a sequence having an identity of at least 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence as set forth in SEQ ID NO: 4, preferably over the entire length of the sequence;
mutating endogenous Zm00001d049145 to a sequence encoding a sequence as set forth in SEQ ID NO: 6 or a sequence having an identity of at least 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence as set forth in SEQ ID NO: 6, preferably over the entire length of the sequence;
mutating endogenous Zm00001d049148 to a sequence encoding a sequence as set forth in SEQ ID NO: 8 or a sequence having an identity of at least 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence as set forth in SEQ ID NO: 8, preferably over the entire length of the sequence; and/or
mutating endogenous Zm00001d049159 to a sequence encoding a sequence as set forth in SEQ ID NO: 10 or a sequence having an identity of at least 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence as set forth in SEQ ID NO: 10, preferably over the entire length of the sequence.

### Claim 11 (depends on 2)

A maize plant comprising a QTL allele located on chromosome 4 in a maize plant or plant part, wherein said QTL allele is located on a chromosomal interval flanked by marker alleles A and B, wherein marker alleles A and B are SNPs which are respectively adenine (A) at the position 16107190 bp on reference genome B73 AGPv04 and cytosine (C) at the position 18470371 bp on reference genome B73 AGPv04, one or more marker allele selected from M1 to M57 as defined in claim 2, one or more polynucleic acid having a coding sequence selected from SEQ ID NOs: 1, 3, 5, 7, 9 and 38, one or more polynucleic acid having a coding sequence with an identity of at least 80%, 85%, 90%, 95%, 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence selected from SEQ ID NOs: 1, 3, 5, 7, 9 and 38, preferably over the entire length of the sequence, one or more polynucleic acid encoding a sequence selected from SEQ ID NOs: 2, 4, 6, 8, 10 and 39 and/or one or more polynucleic acid encoding a sequence with an identity of at least 80%, 85%, 90%, 95%, 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% to a sequence selected from SEQ ID NOs: 2, 4, 6, 8, 10 and 39, preferably over the entire length of the sequence, or a plant part thereof, or the progeny thereof.

### Claim 12 (depends on 11)

The maize plant or plant part according to claim 11, which is transgenic, gene-edited, or mutagenized.

### Claim 13

An isolated polynucleic acid comprising a coding sequence selected from SEQ ID NOs: 1, 3, 5, 7, 9 and 38 or which is at least 80%, 85%, 90%, 95%, 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% identical, preferably over its entire length, to a sequence of any of SEQ ID NOs: 1, 3, 5, 7, 9 and 38; or which encodes a polypeptide selected from SEQ ID NOs: 2, 4, 6, 8, 10 and 39 or a polypeptide which is at least 80%, 85%, 90%, 95%, 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% identical, preferably over its entire length, to a sequence of any of SEQ ID NOs: 2, 4, 6, 8, 10 and 39.

### Claim 14 (depends on 13)

The isolated polynucleic acid according to claim 13, wherein said polynucleic acid comprises
one or more molecular marker selected from M1 to M12;
one or more molecular marker selected from M13 to M17;
one or more molecular marker selected from M18 to M30;
one or more molecular marker selected from M31 to M48; and/or
one or more molecular marker selected from M49 to M56.

### Claim 15 (depends on 13)

An isolated polynucleic acid specifically hybridizing with the polynucleic acid of claim 13, the complement thereof, or the reverse complement thereof, preferably wherein said polynucleic acid is a primer or a probe.