# US 2023/0054088 A1 · Flow Cell and Automatic Analysis Device > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2023/0054088 A1 - Document ID: US20230054088A1 - Status: Published application - Kind: A1 - Application number: 17/792,519 - Filed: 2020-11-27 - Published: 2023-02-23 - Earliest claimed: 2020-01-30 - Assignees: Hitachi High-Tech Corporation - Applicants: Hitachi High-Tech Corporation - Inventors: Yoshinori NEGISHI; So OGUCHI; Yoshiaki SUGIMURA; Yuri KAJIHARA - Web page: https://patentgrep.com/patent/US20230054088A1 ## CPC - G01N 21/05: Flow-through cuvettes (G01N21/09 takes precedence; handling fluid samples G01N1/10) - G01N 21/76: Chemiluminescence; Bioluminescence - G01N 33/543: with an insoluble carrier for immobilising immunochemicals - G01N 35/08: using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis - G01N 21/66: electrically excited, e.g. electroluminescence - G01N 33/483: Physical analysis of biological material - G01N 35/0098: {involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation (magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C)} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Provided are a flow cell and an automatic analysis device which are excellent in the long-term stability of the flow path shape of the flow cell and can improve the reproducibility of analysis. The flow cell (6) of the present invention includes a base (18) including an inlet and outlet (35, 36) for a flow path of a reaction solution containing an analyte; an electrode (16A, 16B) for applying a voltage to the analyte; a light receiving window (22) made of a member that transmits light emitted from the analyte by the voltage applied on the electrode, a gasket (18A) provided between the base (18) and the light receiving window (22), and a flow chamber (17) surrounded by the base (18), the light receiving window (22) and the gasket (18A), in which the gasket (18A) has a deformation amount of 0 mm or more and 0.2 mm or less in a chemical immersion test, and the surface adhesive force of 14 N/cm2 or more and 40 N/cm2 or less in a surface adhesion evaluation ## Claims ### Claim 1 A flow cell comprising: a base provided with an inlet and outlet of a flow path of a fluid containing an analyte, an electrode for applying a voltage to the analyte, a light receiving window made of a member that transmits light emitted from the analyte by the voltage applied on the electrode, a gasket provided between the base and the light receiving window, and a flow chamber surrounded by the base, the light receiving window and the gasket, wherein the gasket has a deformation amount of 0 mm or more and 0.2 mm or less in the chemical immersion test defined in the following (1), and a surface adhesive force of 14 N/cm2 or more and 40 N/cm2 or less in the surface adhesion evaluation test defined in the following (2): (1) Chemical immersion test: After immersion in 0.2 mol/L of potassium hydroxide solution at 40° C. for 31 days, the amount of dimensional change is measured. (2) Surface adhesion evaluation test: A lower surface of a test piece (10 mm square, 0.5 mm thick) is fixed to acrylic resin 1 (20 mm×30 mm, 2 mm thick) with double-sided tape; acrylic resin 2 (20 mm×60 mm, 2 mm thick) is placed on an upper surface of the test piece; a 2 kg weight is allowed to stand on the upper surface of the acrylic resin 2 for 1 minute; the acrylic resin 2 of the evaluation sample obtained by integrating the acrylic resin 1, the test piece, and the acrylic resin 2 is moved in the vertical direction; and the force at which the interface between the test piece and the acrylic resin 2 separates is measured with a force gauge. ### Claim 2 (depends on 1) The flow cell according to claim 1, wherein the deformation amount is 0.1 mm or less. ### Claim 3 (depends on 1) The flow cell according to claim 1, wherein the deformation amount is 0.03 mm or less. ### Claim 4 (depends on 1) The flow cell according to claim 1, wherein the surface adhesive force is 15 N/cm2 or more and 40 N/cm2 or less. ### Claim 5 (depends on 1) The flow cell according to claim 1, wherein the surface adhesive force is 15 N/cm2 or more and 17 N/cm2 or less. ### Claim 6 (depends on 1) The flow cell according to claim 1, wherein the material of the gasket is fluoro rubber. ### Claim 7 (depends on 6) The flow cell according to claim 6, wherein the material of the gasket is a ternary fluoro rubber composed of hexafluoropropylene/vinylidene fluoride/tetrafluoroethylene. ### Claim 8 An automatic analysis device comprising: a flow cell including a base provided with an inlet and outlet of a flow path of a fluid containing an analyte, an electrode for applying a voltage to the analyte, a light receiving window made of a member that transmits light emitted from the analyte by the voltage applied on the electrode, a gasket provided between the base and the light receiving window, and a flow chamber surrounded by the base, the light receiving window, and the gasket; a unit for supplying a fluid containing magnetic particles having a labeling substance to the flow cell; a voltage applying unit for applying a voltage to the electrode; a magnetic trapping unit for trapping the magnetic particles supplied to the flow cell at a predetermined location; and a light detection unit for detecting light generated from the flow cell via the light receiving window, wherein the gasket has a deformation amount of 0 mm or more and 0.2 mm or less in the chemical immersion test defined in the following (1), and the gasket has a surface adhesive force of 14 N/cm2 or more and 40 N/cm2 or less in the surface adhesion evaluation test defined in the following (2): (1) Chemical immersion test: After immersion in 0.2 mol/L of potassium hydroxide solution at 40° C. for 31 days, the amount of dimensional change is measured. (2) Surface adhesion evaluation test: A lower surface of a test piece (10 mm square, 0.5 mm thick) is fixed to acrylic resin 1 (20 mm×30 mm, 2 mm thick) with double-sided tape; acrylic resin 2 (20 mm×60 mm, 2 mm thick) is placed on an upper surface of the test piece; a 2 kg weight is allowed to stand on the upper surface of the acrylic resin 2 for 1 minute; the acrylic resin 2 of the evaluation sample obtained by integrating the acrylic resin 1, the test piece, and the acrylic resin 2 is moved in the vertical direction; and the force at which the interface between the test piece and the acrylic resin 2 separates is measured with a force gauge. ### Claim 9 (depends on 8) The automatic analysis device according to claim 8, wherein the deformation amount is 0.1 mm or less. ### Claim 10 (depends on 8) The automatic analysis device according to claim 8, wherein the deformation amount is 0.03 mm or less. ### Claim 11 (depends on 8) The automatic analysis device according to claim 8, wherein the surface adhesive force is 15 N/cm2 or more and 40 N/cm2 or less. ### Claim 12 (depends on 8) The automatic analysis device according to claim 8, wherein the surface adhesive force is 15 N/cm2 or more and 17 N/cm2 or less. ### Claim 13 (depends on 8) The automatic analysis device according to claim 8, wherein the material of the gasket is fluoro rubber. ### Claim 14 (depends on 8) The automatic analysis device according to claim 8, wherein the material of the gasket is a ternary fluoro rubber composed of hexafluoropropylene/vinylidene fluoride/tetrafluoroethylene.