# US 2023/0053472 A1 · Antimicrobial peptides > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2023/0053472 A1 - Document ID: US20230053472A1 - Status: Published application - Kind: A1 - Application number: 17/932,316 - Filed: 2022-09-15 - Published: 2023-02-23 - Earliest claimed: 2017-07-14 - Assignees: MATERIAS S.r.l. - Applicants: MATERIAS S.r.l. - Inventors: Marco Balestrieri; Gianna Palmieri; Gianluca Neglia; Aniello Anastasio; Federico Capuano; Luca De Stefano; Luigi Nicolais - Web page: https://patentgrep.com/patent/US20230053472A1 ## CPC - A61K 38/10: Peptides having 12 to 20 amino acids {(A61K38/043 - A61K38/046 take precedence)} - A01N 37/46: N-acyl derivatives - A01N 63/50: Isolated enzymes; Isolated proteins (peptides A01N37/46) - A61K 47/6929: {the form being a nanoparticle, e.g. an immuno-nanoparticle} - A61P 31/04: Antibacterial agents - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS ## Abstract The present invention relates to the use of at least one peptide having sequence VRLIVAVRIWRR, VRLIVAVRIKRR, VRLIVKVRIWRR, VRLIVKVRIKRR (SEQ ID NO: 1, SEQ ID NO 2, SEQ ID NO 3, SEQ ID NO 4), as a bactericidal antimicrobial agent against Listeria monocytogenes. ## Claims ### Claim 1 A method for the prevention and/or treatment of contamination of a product or a surface by Listeria monocytogenes, the method comprising contacting the product or surface with a mixture of at least two peptides selected from the group consisting of VRLIVAVRIWRR (SEQ ID NO: 1), VRLIVAVRIKRR (SEQ ID NO: 2), VRLIVKVRIWRR (SEQ ID NO: 3), and VRLIVKVRIKRR (SEQ ID NO: 4), or a salt or solvate thereof. ### Claim 2 (depends on 1) The method according to claim 1, wherein said mixture comprises at least one peptide selected from the group consisting of VRLIVAVRIWRR (SEQ ID NO: 1) and VRLIVAVRIKRR (SEQ ID NO: 2) and at least one peptide selected from the group consisting of VRLIVKVRIWRR (SEQ ID NO: 3) and VRLIVKVRIKRR (SEQ ID NO: 4). ### Claim 3 (depends on 2) The method according to claim 2, wherein said mixture comprises the peptide VRLIVAVRIWRR (SEQ ID NO: 1) and the peptide VRLIVKVRIWRR (SEQ ID NO: 3). ### Claim 4 (depends on 1) The method according to claim 1, wherein said Listeria monocytogenes is an antibiotic-resistant Listeria monocytogenes serotype. ### Claim 5 (depends on 1) The method according to claim 1, wherein said mixture of at least two peptides is not in association with other bactericidal compounds. ### Claim 6 (depends on 1) The method according to claim 1, wherein said product is a food, a material or an object. ### Claim 7 (depends on 1) The method according to claim 1, wherein said mixture of at least two peptides is applied as a coating composition on the surface of said product or on said surface or linked with a covalent bond to said product or surface. ### Claim 8 A method for the treatment of an infection from Listeria monocytogenes in a subject in need thereof, said method comprising administering a mixture of at least two peptides, wherein the peptides have the amino acid sequence selected from the group consisting of VRLIVAVRIWRR (SEQ ID NO: 1), VRLIVAVRIKRR (SEQ ID NO: 2), VRLIVKVRIWRR (SEQ ID NO: 3), and VRLIVKVRIKRR (SEQ ID NO: 4), or a pharmaceutically acceptable salt or solvate thereof. ### Claim 9 (depends on 8) The method according to claim 8, wherein said Listeria monocytogenes is an antibiotic-resistant Listeria monocytogenes serotype. ### Claim 10 (depends on 8) The method according to claim 8, wherein said mixture of at least two peptides is a mixture between at least one peptide selected from the group consisting of VRLIVAVRIWRR (SEQ ID NO: 1) and VRLIVAVRIKRR (SEQ ID NO: 2) and at least one peptide selected from the group consisting of VRLIVKVRIWRR (SEQ ID NO: 3) and VRLIVKVRIKRR (SEQ ID NO: 4). ### Claim 11 Antimicrobial composition or pharmaceutical composition comprising a mixture of at least one peptide selected from the group consisting of VRLIVAVRIWRR (SEQ ID NO: 1) and VRLIVAVRIKRR (SEQ ID NO: 2) and at least one peptide selected from the group consisting of VRLIVKVRIWRR (SEQ ID NO: 3) and VRLIVKVRIKRR (SEQ ID NO: 4). ### Claim 12 Product having at least one surface linked to at least two peptides selected from the group consisting of VRLIVAVRIWRR (SEQ ID NO: 1), VRLIVAVRIKRR (SEQ ID NO: 2), VRLIVKVRIWRR (SEQ ID NO: 3), and VRLIVKVRIKRR (SEQ ID NO: 4), or a salt or solvate thereof, wherein said at least two peptides are linked to said surface by means of a covalent bond between N-terminal amino group of the peptide and a chemical group on said surface. ### Claim 13 Nanoparticles comprising, covalently linked to their surface, at least two peptides selected from the group consisting of VRLIVAVRIWRR (SEQ ID NO: 1), VRLIVAVRIKRR (SEQ ID NO: 2), VRLIVKVRIWRR (SEQ ID NO: 3), and VRLIVKVRIKRR (SEQ ID NO: 4). ### Claim 14 (depends on 12) Product as claimed in claim 12 or nanoparticles comprising, covalently linked to their surface, at least two peptides selected from the group consisting of VRLIVAVRIWRR (SEQ ID NO: 1), VRLIVAVRIKRR (SEQ ID NO: 2), VRLIVKVRIWRR (SEQ ID NO: 3), and VRLIVKVRIKRR (SEQ ID NO: 4), wherein said at least two peptides comprise at least one peptide selected from the group consisting of VRLIVAVRIWRR (SEQ ID NO: 1) and VRLIVAVRIKRR (SEQ ID NO: 2) and at least one peptide selected from the group consisting of VRLIVKVRIWRR (SEQ ID NO: 3) and VRLIVKVRIKRR (SEQ ID NO: 4). ### Claim 15 (depends on 13) Nanoparticles as claimed in claim 13, having an average hydrodynamic diameter measured by dynamic light scattering (DLS) comprised between 5 and 90 nm. ### Claim 16 (depends on 13) Nanoparticles as claimed in claim 13, which are hybrid nanoparticles. ### Claim 17 (depends on 12) Product as claimed in claim 12 or nanoparticles comprising, covalently linked to their surface, at least two peptides selected from the group consisting of VRLIVAVRIWRR (SEQ ID NO: 1), VRLIVAVRIKRR (SEQ ID NO: 2), VRLIVKVRIWRR (SEQ ID NO: 3), and VRLIVKVRIKRR (SEQ ID NO: 4), wherein the N-terminal amino group of said peptides is covalently bound to a chemical group on said surface selected from carboxyl groups, excited hydroxyl radicals, activated alkoxy groups or activated aldehyde or ketone groups. ### Claim 18 (depends on 1) The method according to claim 1, wherein said peptides are covalently linked to the surface of nanoparticles. ### Claim 19 (depends on 8) The method according to claim 8, wherein said peptides are covalently linked to the surface of nanoparticles.