# US 2023/0053333 A1 · Preparation method of crystal structure analysis sample for structural analysis using crystal sponge method

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- Number: US 2023/0053333 A1
- Document ID: US20230053333A1
- Status: Published application
- Kind: A1
- Application number: 17/778,531
- Filed: 2020-11-20
- Published: 2023-02-23
- Earliest claimed: 2019-11-21
- Assignees: Kirin Holdings Kabushiki Kaisha
- Applicants: Kirin Holdings Kabushiki Kaisha
- Inventors: Yoshimasa TANIGUCHI; Rie MATSUMOTO
- Web page: https://patentgrep.com/patent/US20230053333A1

## CPC

- G01N 1/28: Preparing specimens for investigation {including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q}(mounting specimens on microscopic slides G02B21/34; means for supporting the objects or the materials to be analysed in electron microscopes H01J37/20 {; laboratory gas handling apparatus B01L5/00})
- G01N 23/207: Diffractometry using detectors, e.g. using a probe in a central position and one or more displaceable detectors in circumferential positions
- G01N 2223/05: by diffraction, scatter or reflection
- G01N 2223/079: incident electron beam and measuring excited X-rays
- G01N 2223/605: phases
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

An object of the present invention is to provide a method of preparing a sample for crystallographic analysis used for structure determination based on the crystalline sponge method. The present invention provides a method of preparing a sample for crystallographic analysis used for structure determination based on the crystalline sponge method, the method including the steps: (A) forming an ionic pair of a target compound of analysis with a counterionic compound, and (B) soaking the ionic pair of the compounds into a crystalline sponge, wherein the target compound of analysis is a basic compound or an acidic compound.

## Claims

### Claim 1

A method of preparing a sample for crystallographic analysis used for structure determination based on the crystalline sponge method, the method comprising the steps: (A) forming an ionic pair of a target compound of analysis with a counterionic compound, and (B) soaking the ionic pair of the compounds into a crystalline sponge, wherein the target compound of analysis is a basic compound or an acidic compound.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the target compound of analysis is a basic compound, and wherein the basic compound forms an ionic pair with an acidic compound in the step (A).

### Claim 3 (depends on 1)

The method according to claim 1, wherein the target compound of analysis is an acidic compound, and wherein the acidic compound forms an ionic pair with a basic compound in the step (A).

### Claim 4 (depends on 1)

The method according to any one of claim 1, wherein the step (A) and the step (B) are performed sequentially or simultaneously.

### Claim 5 (depends on 1)

A method of determining the structure of a target compound of analysis, the method comprising the steps of: performing the preparation method according to any one of claim 1 to prepare a sample for crystallographic analysis; and performing structural analysis of the prepared sample for crystallographic analysis.