Vesicle and use thereof
AssigneeEv Cell Biotech (Guangzhou) Co., Ltd.
InventorsXiao ZHANG, Xiaoxing KOU, Songtao SHI
Life of this patent
Estimating the term…
Abstract
Provided are a vesicle and the use thereof. The vesicle is an induced vesicle, and the sources thereof include stem cells or somatic cells, and the possessed markers include Syntaxin 4. Compared with an exosome in mesenchymal stem cells, the vesicle can specifically express Syntaxin 4 and can be used to distinguish characteristic markers of MSC-derived vesicles and exosomes. The vesicle can play a procoagulant effect in vitro, can improve the bleeding tendency of mice with hemophilia after in vivo injection, and can be used for the treatment of improving the bleeding tendency of hemophilia. In addition, the vesicle can be expelled through the skin and hair.
Claims13
A vesicle, characterized in that, the vesicle is an induced vesicle, the vesicle is derived from sources comprises stem cells or somatic cells, and the vesicle has markers comprising Syntaxin 4.
A reagent or kit for screening or identification or extraction of a vesicle, characterized by comprising detection reagents for one or more of markers Annexin V, Flotillin-1, Cadherin 11, Integrin alpha 5, and Syntaxin 4;
- preferably, the detection reagents detect the expression levels of genes of the markers;
- further preferably, the detection reagents detect the expression levels of mRNAs of the markers;
- more preferably, the detection reagents for the markers detect the expression levels of proteins of the markers;
- or
- preferably, the detection reagents for the markers are one or more of fluorescent quantitative PCR dyes, fluorescent quantitative PCR primers, fluorescent quantitative PCR probes, antibodies, antibody functional fragments, and conjugated antibodies;
- or
- preferably, the kit is selected from one or more of a qPCR kit, an immunoblot assay kit, a flow cytometry assay kit, an immunohistochemical assay kit, and an ELISA kit;
- more preferably, the kit is selected from a flow cytometry assay kit;
- and
- preferably, the vesicle is an induced vesicle.
Dependencies are inferred from the claim text.