Ribosome-Mediated Incorporation of Peptides and Peptidomimetics
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsSidney Hecht, Larisa (Liza) Dedkova, Rumit Maini, Sandipan Roy Chowdhury, Rakesh Paul
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Abstract
Modified ribosomes that were selected using a dipeptidyl-puromycin aminonucleoside are used to mediate site-specific incorporation of one or more peptides and peptidomimetics into protein in a cell free translation system. In addition, new fluorescent dipeptidomimetics have been synthesized and incorporated into proteins, as well as modified proteins containing one or more non-naturally occurring dipeptides.
Claims20
-11. (canceled)
A genetically modified bacterial ribosome, the modification consisting of an Escherichia coli (E. coli) 23 S ribosomal RNA (rRNA) sequence modification, the 23 S rRNA sequence modification consisting of a modification in Region 1 (position 2057-2063) selected from UGCGUGG or AGCGUGA, and a modification in Region 2 (position 2502-2507), wherein:
- (a) if the modification in Region 1 consists of UGCGUGG from position 2057-2063, the modification in Region 2 is selected from the group consisting of ACGAAG, CGCACG, CUAUGU, CGCAAU, or CUACAG from position 2502-2507;
- (b) if the modification in Region 1 consists of AGCGUGA from position 2057-2063, the modification in Region 2 is selected from the group consisting of CUGCGU, UGGCAG, AUCAGG, or AUCCGA from position 2502-2507.
Dependencies are inferred from the claim text.